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Flot1 (NM_008027) Mouse Untagged Clone

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SKU
MC200016
Flot1 (untagged) - Mouse flotillin 1 (Flot1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $686.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Flot1
Synonyms reggie-2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC004647 sequence for NM_008027
GTCTGTTGCTGCAACAGGGTGCGGCCGGGTGGGGGGCGCAAGTGCAGCTTCCACTAAGCCGGGTGTCCGA GCAGTCCCCACCCCGTTCTGGGGTTCTGCGGGCCTCCGCGCTTCTCAAGACCCCGGTGGGGTTGCTTACC GGGACGTTCCGGAAGCTGCAGCTTCAACCATGTTTTTCACTTGTGGCCCAAATGAGGCCATGGTGGTCTC CGGGTTCTGCAGGAGCCCCCCAGTCATGGTGGCCGGAGGCCGAGTGTTTGTCCTACCCTGCATTCAGCAA ATCCAGAGGATCTCTCTCAACACACTGACCCTCAATGTCAAGAGTGAAAAGGTTTATACCCGCCACGGGG TCCCCATCTCAGTCACGGGCATTGCCCAGGTGAAAATCCAGGGCCAGAACAAGGAGATGCTGGCAGCTGC CTGCCAGATGTTCCTGGGGAAGACAGAGGCGGAGATTGCCCACATTGCCCTGGAGACACTGGAAGGCCAC CAGAGGGCAATCATGGCCCACATGACTGTGGAGGAGATCTATAAGGATAGACAGAAATTCTCAGAGCAGG TTTTCAAAGTGGCCTCCTCCGACCTGGTCAACATGGGTATCAGCGTGGTTAGCTACACTTTGAAGGATAT TCATGATGACCAGGATTACTTACACTCGTTAGGAAAGGCTCGCACGGCACAAGTCCAAAAAGATGCTCGG ATTGGCGAAGCAGAGGCCAAGAGAGATGCCGGGATACGGGAGGCTAAGGCCAAGCAGGAGAAGGTATCTG CACAGTGCCTCAGTGAAATAGAGATGGCCAAGGCACAGAGGGACTATGAGCTGAAGAAAGCCACCTACGA CATCGAGGTCAACACCCGGCGAGCACAGGCTGACCTGGCCTATCAGCTGCAGGTGGCCAAGACGAAGCAG CAGATCGAGGAGCAGCGGGTGCAGGTGCAGGTGGTTGAGCGCGCCCAGCAGGTGGCAGTGCAGGAGCAGG AGATCGCCCGGCGCGAGAAGGAGTTGGAGGCTCGCGTGCGCAAGCCAGCAGAGGCTGAGCGCTACAGGCT GGAGCGCCTGGCGGAAGCAGAGAAGGCCCAGCTGATCATGCAGGCAGAAGCCGAAGCTGAGTCTGTGCGG ATGCGAGGGGAAGCTGAGGCCTTTGCTATAGGTGCCCGCGCTCGTGCTGAGGCTGAGCAAATGGCCAAGA AGGCAGAAGCCTTCCAGATGTACCAGGAGGCCGCCCAGCTGGACATGCTGCTGGAGAAACTGCCCCAGGT GGCAGAAGAGATCAGTGGTCCCCTGACCTCAGCCAATAAGATCACGCTGGTGTCTAGTGGAAGCGGAACC ATGGGGGCAGCCAAAGTTACTGGGGAAGTACTGGACATCTTGAGCCGCCTGCCGGAGAGTGTGGAGAGGC TCACAGGCGTCAGCATCTCCCAGGTAAATCACAACAAGCCTTTAAGGACAGCATGAGCCCTCAGTCCTGG ACTGTGTGGCCTGAGCGGTCTCATTGGGGAGTGGTCTCATCGCATGCACCTCACCTGGCCTCCCTTAGCA TGGCCCCCGACAGGGGTGCACACCTCTCCTTGCCAAATAGTCCGTGCCTTGCCTTGGAGGGTTTGCCCTT CTTAGCCCATTGCCAGTGCCCTGCACCAAGATTGATTATCTTCAGCCCATCCCAACTATTTAACTTCCTG ATTAAACTAGACTGTTTGAGCCTGAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_008027
Insert Size 1287 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC004647, AAH04647
RefSeq Size 1721 bp
RefSeq ORF 1287 bp
Locus ID 14251
UniProt ID O08917
Cytogenetics 17 B1
Summary May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008027" in other vectors (4)
SKU Description Size Price
MG206823 Flot1 (tGFP-tagged) - Mouse flotillin 1 (Flot1) 10 ug
$886.00
MR206823 Flot1 (Myc-DDK-tagged) - Mouse flotillin 1 (Flot1) 10 ug
$686.00
MR206823L3 Lenti ORF clone of Flot1 (Myc-DDK-tagged) - Mouse flotillin 1 (Flot1) 10 ug
$986.00
MR206823L4 Lenti ORF clone of Flot1 (mGFP-tagged) - Mouse flotillin 1 (Flot1) 10 ug
$986.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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