Rabbit polyclonal eGFP antibody

CAT#: TA150032

Rabbit polyclonal eGFP antibody

Product Images

Western blot analysis of 1 ug of whole cell lysate with anti-GFP antibody TA150032 diluted at 1:10000. A: Non-transfected 293T lysates; B: GFP transfected 293T lysates; C: RFP transfected 293T lysates.

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  GFP antibody detects GFP protein by Western blot analysis. Non-transfected (-) and GFP-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (TA150032) diluted at 1:10000.

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  Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo (fixed with ice-cold acetone), using GFP (TA150032) antibody at 1:200 dilution.

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  Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( TA150032, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

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  GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (TA150032, 1:15000).

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1:5000-1:20.000
  ICC/IF: 1:1000-1:5000
  IP: 1:100-1:500
  IHC Whole Mount: 1:100-1:500
• Target: eGFP
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length protein of eGFP (1-265aa)
• Isotype: IgG
• Formulation: PBS (pH7.0) containing 1% BSA, 20% glycerol and 0.025% ProClin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
• Stability: Stable for 1 year at -20°C from date of shipment.
• Predicted Protein Size: 29.0 kDa