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Carrier-free (BSA/glycerol-free) DDK (FLAG) mouse monoclonal antibody, clone OTI11C3

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SKU
CF180144
Carrier-free (BSA/glycerol-free) DDK (FLAG) mouse monoclonal antibody, clone OTI11C3
  $620.00
3 Days*
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Specifications
Specifications
Product Data
Clone Name OTI11C3
Application ICC, IF, WB
Recommended Dilution WB 1:4000
Reactivity DDK (FLAG tag): DYKDDDDK
Antibody Host Mouse
Clonality Monoclonal
Immunogen Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
Isotype IgG1
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Conjugation Unconjugated
Storage Store at -20°C.
Stability Stable for 1 year at -20°C from date of shipment.
Shipping Ambient
Note This anti-DDK (FLAG) antibody recognizes both N-terminus and C-terminus DDK tags.
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Other "OTI11C3" antibodies (2)
SKU Description Size Price
TA180144 DDK (FLAG) mouse monoclonal antibody, clone OTI11C3 100 ul
$447.00
TA180144S DDK (FLAG) mouse monoclonal antibody, clone OTI11C3 30 ul
$200.00

file-alt Citations

The circadian gene Dec2 promotes pancreatic cancer progression and dormancy through immune evasion
Wang, Harris, Dudgeon et al
Dev Cell (2026)
Cited by 2
AI Summary: OriGene’s DDK (FLAG) mouse monoclonal antibody (TA180144) was used in this study to detect tagged proteins, contributing to the investigation of Dec2’s role in pancreatic cancer progression and immune evasion.
Snippet: Anti-mCherry Antibody Rockland 600-401-P16S;AB_2209751 Anti-Ki67 (D3B5) Rabbit mAb Cell Signaling Technology 9129;AB_2687446 DDK (FLAG) mouse monoclonal antibody Origene TA180144 Anti-Ki-67 Antibody (Ki-S5) Sigma-Aldrich MAB4190;AB_95092
Exome Sequencing Reveals the Genetic Architecture of Non-syndromic Orofacial Clefts and Identifies BOC as a Novel Causal Gene
He, Yu, Jiao et al
Adv Sci (Weinh) (2025)
Cited by 4
AI Summary: In this study, OriGene's FLAG antibody (TA180144) and β-actin antibody (TA811000) were used for Western blotting and immunofluorescence analyses to assess protein expression and validate the functional impact of BOC and GLI2 variants in nonsyndromic orofacial clefts.
Snippet: Western blotting, and Immunofluorescence (IF) were performed as described previously.[30] The antibodies used were PTCH1 (CST, #2468S), HA (Proteintech, # 51064-2-AP), FLAG (Origene, #TA180144) and β-actin (Origene, #TA811000). Pull-Down Assay
WB IF
Enhanced radiotherapy susceptibility in NSCLC through palbociclib-mediated PP5 inhibition
Huang, Chen, Chen et al
Biochim Biophys Acta Mol Cell Res (2024) 1872 (2), 119884 DOI: 10.1016/j.bbamcr.2024.119884
AI Summary: In this study, OriGene's Mouse anti-DDK antibody (TA180144) was used to detect tagged proteins, contributing to the investigation of PP5's role in enhancing radiotherapy susceptibility in NSCLC.
Snippet: Mouse anti-DDK (TA180144) and FITC-anti-γ-H2AX (Ser139) antibody (#613404) and its isotype control (#400110) were purchased from OriGene Technologies, Inc. (Rockville, MD, USA) and BioLegend (San Diego, CA, USA), respectively. Secondary antibodies anti-rabbit-HRP (111-035-003), anti-mouse-HRP (115-035-003), and anti-goat-HRP (112-003-035) were from Jackson ImmunoResearch (West Grove, PA, USA)
The circadian gene Dec2 promotes pancreatic cancer dormancy by regulating tumor cell antigen presentation to facilitate immune evasion
Harris, Wang, Dudgeon et al
bioRxiv (2024)
Snippet: Anti-mCherry Antibody Rockland 600-401-P16S Anti-Ki67 (D3B5) Rabbit mAb Cell Signaling Technology 9129 DDK (FLAG) mouse monoclonal antibody Origene TA180144 Anti-Ki-67 Antibody (Ki-S5) Sigma-Aldrich MAB4190
Progerin Inhibits the Proliferation and Migration of Melanoma Cells by Regulating the Expression of Paxillin
Liu, Huang, Luo et al
Onco Targets Ther (2024) 17, 227-242 DOI: 10.2147/OTT.S442504
AI Summary: OriGene's Flag antibody (#TA180144) was used in this study to detect Flag-tagged proteins in Western blot experiments, contributing to the analysis of progerin and paxillin expression in melanoma cells.
Snippet: was blocked with 5% milk in TBST and then incubated with specific primary antibodies: LaminA/C (1:1000, ab169532, Abcam, USA), Paxillin (1:1000, #2542, Cell Signaling, USA), Flag (1:1000, #TA180144, OriGene, USA), N-cadherin (1:2000, 66219-1-Ig, Proteintech, USA), E-cadherin (1:5000, 20874-1-AP, Proteintech, China), vimentin (1:10000, 60330-1-1g, Proteintech, USA), and GAPDH (1:10000, 60004-1-Ig,
FigureFigureFigure
WB
SPTSSA variants alter sphingolipid synthesis and cause a complex hereditary spastic paraplegia
Srivastava, Shaked, Gable et al
Brain (2023)
Cited by 37
AI Summary: OriGene products contributed to this study by providing plasmids for expression of SPTLC1 and SPTLC2, which were used to investigate the effects of SPTSSA variants on serine palmitoyltransferase function and sphingolipid synthesis.
Snippet: Sphingolipids are a diverse family of lipids with critical structural and signalling functions in the mammalian nervous system, where they are abundant in myelin Save;Plasmids for expression of SPTLC1 and SPTLC2 were purchased from OriGene. Plasmids for expressing NubG-HA-tagged SPTSSA and Nu
Showing 1-6 of 27 papers.
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