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GFP Goat Polyclonal Antibody

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SKU
AB0020-500
Goat Polyclonal Anti-GFP Antibody
Size:
1 mg 400 ug 20 ul
Conjugation:
Unconjugated
  $705.00
In Stock*
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Specifications
Specifications
Product Data
Application IF, WB
Recommended Dilution WB 1:500-1:5,000; IF 1:50-1:1,000; IHC (paraffin) 1:50-1:1,000; IHC (frozen) 1:50-1:1,000
Reactivity GFP tag
Antibody Host Goat
Clonality Polyclonal
Immunogen Purified recombinant peptide produced in E. coli.
Isotype IgG
Formulation Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration 2mg/ml
Purification epitope affinity purified
Conjugation Unconjugated
Shipping Blue Ice
Background Green Fluorescent Protein
Note In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.
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Documents
Resources

file-alt Citations

Trithorax balances ISC fate decisions via Ptx1-mediated repression of EE specification
Fasoulaki, Hatzis, Delidakis et al
bioRxiv (2025)
AI Summary: In this study, the OriGene goat anti-GFP antibody (AB0020-500) was used for immunostaining to detect GFP-labeled proteins, aiding in the analysis of intestinal stem cell fate in Drosophila.
Snippet: (C594.9B, 1:100, DSHB), rabbit anti-GFP (Minotech, Cat# 701‐1, 1:10000) and goat anti-GFP (Origene AB0020-500). Secondary antibodies Alexa Fluor 488 Goat anti-Rabbit IgG (Thermo Fisher Scientific Cat# A-11034, RRID:AB_2576217) , Alexa Fluor 555 Goat antiMouse IgG (Molecular Probes C
Transduction of Surface and Basal Cells in Rhesus Macaque Lung Following Repeat Dosing with AAV1CFTR
Guggino, Yanda, Cebotaru et al
Human gene therapy (2020) 31 (17-18), 1010-1023 DOI: 10.1089/hum.2020.207
Cited by 14
AI Summary: OriGene’s anti-GFP antibody (#AB0020-500) was used for Western blot detection of GFP protein to assess transduction efficiency in monkey lung tissues in this study.
Snippet: GFP was IP with anti-GFP antibody (#11814460001;Roche) and incubated with A/G beads for 4 h at 4°C. Western blotting was performed, and samples were incubated with the anti-GFP primary antibody (#AB0020-500;Origene)
IP WB
Opposing expression gradients of calcitonin-related polypeptide alpha (Calca/Cgrpα) and tyrosine hydroxylase (Th) in type II afferent neurons of the mouse cochlea
Wu, Vyas, Glowatzki et al
J Comp Neurol (2018) 526 (3), 425-438 DOI: 10.1002/cne.24341
Cited by 32
AI Summary: In this study, OriGene products, specifically the anti-Tyrosine Hydroxylase antibody (TA324946) and anti-Calcitonin Gene Related Peptide (CGRP) antibody (TA326083), were used for immunolabeling to visualize and compare the expression patterns of Th and Calca/Cgrpα in mouse cochlear type II afferent neurons.
FigureFigure
Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes
Agarwal, Wu, Hughes et al
Neuron (2017) 93 (3), 587-605.e7 DOI: 10.1016/j.neuron.2016.12.034
Cited by 473
AI Summary: In this study, OriGene's SOD1 G93A plasmid was used to express the ALS-associated mutant superoxide dismutase 1 in astrocytes, enabling the authors to investigate how this mutation enhances mitochondrial calcium transients and contributes to astrocyte dysfunction.
FigureFigure
Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice
Vyas, Wu, Zimmerman et al
J Assoc Res Otolaryngol (2017) 18 (1), 139-151 DOI: 10.1007/s10162-016-0591-7
Cited by 30
AI Summary: In this study, OriGene’s anti-peripherin antibody (Cat# AM06329PU-N) was used to label and identify type II cochlear afferents, helping to confirm the selectivity of TH-2A-CreER neurons as markers for these fibers.
ELYS regulates the localization of LBR by modulating its phosphorylation state
Mimura, Takagi, Clever et al
J Cell Sci (2016) 129 (22), 4200-4212 DOI: 10.1242/jcs.190678
Cited by 35
AI Summary: In this paper, OriGene products such as anti-LBR antibody (Cat# TA326514) and LBR cDNA clone (Cat# RC204307) were used for immunofluorescence analysis and overexpression experiments, respectively, to investigate how ELYS regulates the localization and phosphorylation state of the Lamin B receptor (LBR).
FigureFigureFigure
Showing 1-6 of 12 papers.
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