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GFP Goat Polyclonal Antibody

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SKU
AB0020-20
Anti GFP
Size:
20 ul 400 ug 1 mg
Conjugation:
Unconjugated
  $99.00
In Stock*
Bulk/Customize

Other products for "GFP"

Specifications
Specifications
Product Data
Application IF, IHC, WB
Recommended Dilution WB 1:500-1:5,000; IF 1:50-1:1,000; IHC (paraffin) 1:50-1:1,000; IHC (frozen) 1:50-1:1,000
Reactivity GFP Tag
Antibody Host Goat
Clonality Polyclonal
Immunogen Purified recombinant peptide produced in E. coli.
Isotype IgG
Formulation Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration 2.0 mg/ml
Purification Epitope Affinity Chromatography
Conjugation Unconjugated
Storage Store at -20°C for long-term storage. Store at 2-8°C for up to one month.
Shipping Blue Ice
Background Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Note In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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