GFP Goat Polyclonal Antibody

Snippet: d GFAP (Supplementary Fig. 9) was conducted as reported previously46. Briefly, sections were pretreated as described above, and then incubated with goat polyclonal anti-GFP antibody (1:500 dilution;OriGene, USA), rabbit monoclonal anti-Iba1 antibody (1:1000 dilution;Wako), mouse monoclonal anti-CD8 antibody (1:1000 dilution;Bio-Rad, USA), anti-NeuN antibody (1:2000 dilution;Millipore, USA), a

Snippet: … Samples corresponding to 0.2 OD600 units of cells were separated by SDS-PAGE, immunobloted to nitrocellulose membrane, and immunodecorated with α-GFP (1:5000, ORIGENE), α-PGK1 (1:10,000, Abcam), or α-HA (1:1,000, Roche). Anti-rabbit, anti-mouse, or anti-rat secondary antibodies conjugated to Dylight 800 (1:40,000, LI-COR) were used and detected with the O;and immunodecorated with α-GFP (1:5000, ORIGENE), α-PGK1 (1:10,000, Abcam), or α-HA (1:1,000, Roche)

Snippet: … Aortic cross sections were stained with antibodies against GFP (green fluorescent protein;Acris Antibodies) and CD31 (Abcam). GFP immunohistochemistry was quantified in the aorta intima using Tissuemorf software (Viopharm). Isometric Tension Studies Mice were euthanized by CO2 inhalation.;- Aortic cross sections were stained with antibodies against GFP (Acris Antibodies) and CD31 (Abcam). GFP immunohistochemistry was quantified in the aorta intima using Tissuemorf software (Viopharm). …

Snippet: pH 7.4, 150 mM NaCl). Then, membranes were probed with diluted anti-GFP (Green fluorescent protein) Ab (polyclonal antibody to GFP-HRP;Acris antibodies GmbH) 1:5000 in blocking solution. Finally, the DAB powder (Sigma;- pH 7.4, 150 mM NaCl). Then, membranes were probed with diluted anti-GFP (Green fluorescent protein) Ab (polyclonal antibody to GFP-HRP