Mouse IgG + IgM (H+L chain), adsorbed Goat Polyclonal Antibody

SKU R1469F

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Reactivity: Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG and IgM whole molecules
• Specificity: This product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative.; Label: FITC; State: Lyophilized purified IgG fraction.; Label: Fluorescein isothiocyanate (MW 390 daltons); Absorption emission: 495 nm / 528 nm; Molar radio: 3.8 moles FITC per mole of Goat IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
• Shipping: Ambient