Mouse IgG + IgM (H+L chain), adsorbed Goat Polyclonal Antibody

SKU R1469HRP

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, HRP

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1/40,000.; Western Blot: 1/2,000-1/10,000.; Immunohistochemistry: 1/500-1/2,500.
• Reactivity: Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG and IgM whole molecules.
• Specificity: This product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Mouse IgG and Mouse IgM.; No reaction was observed against Bovine, Horse or Human serum proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.; Label: HRP; State: Lyophilized purified IgG fraction.; Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient