RAB7 (RAB7A) Rabbit Polyclonal Antibody

CAT#: TA337011

Rabbit Polyclonal Rab7a Antibody

Product Images

Immunocytochemistry/Immunofluorescence: Rab7a Antibody TA337011 - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: Rab7a Antibody TA337011 - Analysis of human Rab7 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse NIH 3T3 and 4) RAW using Rab7 at 0.25 ug/ml.

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  Immunocytochemistry/Immunofluorescence: Rab7a Antibody TA337011 - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry-Paraffin: Rab7a Antibody TA337011 - Analysis of human skeletal muscle using RAB7A antibody at 10 ug/ml.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: Immunohistochemistry, Immunocytochemistry/ Immunofluorescence: 2-5 ug/ml, Immunohistochemistry-Paraffin: 10 ug/ml, Western Blot: 0.5-1 ug/ml
• Reactivities: Human, Mouse, Rat, Bovine, Canine, Chicken, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 90-140 of human Rab7 protein was used as the immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAB7A, member RAS oncogene family
• Database Link:
  NP_004628
  Entrez Gene 29448 RatEntrez Gene 7879 Human
  UniProt ID P51149
• Synonyms: PRO2706; RAB7

Reference Data

• Protein Families: Druggable Genome