BDNF Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:200-1:2000; IHC: 1:100-1:3000 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Peptide (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor). |
| Buffer | Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3. |
| Reconstitution Method | Add 50 ul double distilled water (DDW) to the lyophilized powder. |
| Purification | Affinity purified on immobilized antigen. |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Gene Name | brain-derived neurotrophic factor |
| Database Link | |
| Background | Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways. BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLC? pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system. Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both Kv1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the Nav1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its â??rapidâ? neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission. Due to the high degree of homology between the different neurotrophins it has been difficult to produce truly specific antibodies against any of them. We have now produced a highly specific antibody against BDNF which recognizes exclusively BDNF. |
| Synonyms | ANON2; BULN2 |
| Reference Data | |
| Protein Categories | Growth Factors, Membrane Proteins, Secreated Proteins |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane |
| Protein Pathways | Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
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Citations
The Polyunsaturated Fatty Acid EPA, but Not DHA, Enhances Neurotrophic Factor Expression through Epigenetic Mechanisms and Protects against Parkinsonian Neuronal Cell Death
Ceccarini, Ceccarelli, Codini et al
Int J Mol Sci (2022) 23 (24) DOI: 10.3390/ijms232416176
Ceccarini, Ceccarelli, Codini et al
Int J Mol Sci (2022) 23 (24) DOI: 10.3390/ijms232416176
Cited by 40
AI Summary: In this study, OriGene products—including the BDNF (NM_001709) and GDNF (NM_000514) primers—were used for RT-PCR analysis to measure neurotrophic factor gene expression, contributing to the investigation of how EPA enhances BDNF and GDNF expression through epigenetic mechanisms in neuronal cells.
Effect of amniotic fluid stem cell transplantation on the recovery of bladder dysfunction in spinal cord-injured rats
Liang, Shaw, Ko et al
Sci Rep (2020) 10 (1), 10030 DOI: 10.1038/s41598-020-67163-7
Liang, Shaw, Ko et al
Sci Rep (2020) 10 (1), 10030 DOI: 10.1038/s41598-020-67163-7
Cited by 14
AI Summary: In this study, the OriGene rabbit polyclonal antibody against TGF-β1 was used to detect and analyze TGF-β1 expression in rat bladder tissues, helping to assess molecular changes following amniotic fluid stem cell transplantation in spinal cord-injured rats.
Snippet: EAL peroxidase blocking solution (code S2023, DAKO Corp., Carpinteria, CA, USA) for 20 min. Sections were incubated for 18–20 h at 4 °C with rabbit polyclonal antibodies against TGF-β1 (1:50, OriGene Technologies, Inc., Rockville, MD, USA), β3-adrenoceptor (1:750, Millipore, Temecula, CA), M2 (1:1,000, Millipore, Temecula, CA, USA), M3 (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, U
PGC-1α-FNDC5-BDNF signaling pathway in skeletal muscle confers resilience to stress in mice subjected to chronic social defeat
Zhan, Huang, Li et al
Psychopharmacology (Berl.) (2018)
Zhan, Huang, Li et al
Psychopharmacology (Berl.) (2018)
AI Summary: OriGene contributed to this study by providing the rabbit anti-proBDNF antibody, which was used to measure proBDNF protein levels in mouse skeletal muscle to assess the PGC-1α-FNDC5-BDNF signaling pathway’s role in stress resilience.
Snippet: Cambridge, MA, USA), rabbit anti-mature BDNF (1:500;Sangon Biotech, Shanghai, China), rabbit anti-p-TrkB (1:500;Affinity, Cincinnati, OH, USA), rabbit anti-TrkB (1:1000;Cell Signaling Technology, Danvers, MA, USA), rabbit anti-proBDNF (1:500;Origene, Rockville;Cell Signaling Technology, Danvers, MA, USA), rab- bit anti-proBDNF (1:500
Cocaine-mediated activation of microglia and microglial MeCP2 and BDNF production
Cotto, Li, Tuma et al
Neurobiol. Dis. (2018) 117, 28-41 DOI: 10.1016/j.nbd.2018.05.017
Cotto, Li, Tuma et al
Neurobiol. Dis. (2018) 117, 28-41 DOI: 10.1016/j.nbd.2018.05.017
AI Summary: OriGene's BDNF antibody was used in this study to detect and measure BDNF protein levels, helping to assess how cocaine exposure affects BDNF expression in brain cells.
Snippet: Primary antibodies included: Phospho-MeCP2 pSer421 (1:1000, Thermo Scientific), MeCP2 (1:1000;GeneTex), BDNF (1:1000;Origene), MAP2 (1:1000;Abcam), and the loading controls tubulin (1: 1000;Cell Signaling), lamin (1:1000, Cell Signaling) or GAPDH (1
Blockade of soluble epoxide hydrolase attenuates post-ischemic neuronal hyperexcitation and confers resilience against stroke with TrkB activation
Chang, Lin, Huang et al
Sci Rep (2018) 8 (1), 118 DOI: 10.1038/s41598-017-18558-6
Chang, Lin, Huang et al
Sci Rep (2018) 8 (1), 118 DOI: 10.1038/s41598-017-18558-6
AI Summary: OriGene products, specifically the rabbit anti-BDNF (TA328615) and rabbit anti-GluA2 (TA326000) antibodies, were used for immunohistochemical analysis to detect protein expression changes involved in neuroprotective mechanisms following stroke in this study.
Snippet: The following primary antibodies were then used for incubation overnight at 4 °C: rabbit anti-BDNF (1:500, Origene, TA328615), rabbit anti-phosphorylated TrkB (p-TrkB, 1:500, Abcam, ab109684), rabbit anti-GluN2A (1:1000, Millipore, 05–901 R), rabbit anti-GluN2B (1:1000, Cell Signaling, 4207 S), rabbit anti-p75NTR (1:1000, Abcam, ab52987), rabbit anti-GluA2 (1:1000, Origene, TA326000) and rabbit anti-β-Actin (1:2000, ThermoFisher, PA1-183)
Protective Effect of Hyperbaric Oxygen on Cognitive Impairment Induced by D-Galactose in Mice
Chen, Li, Chen et al
Neurochem. Res. (2016)
Chen, Li, Chen et al
Neurochem. Res. (2016)
Cited by 60
AI Summary: OriGene’s rabbit BDNF monoclonal antibody was used in this study to detect BDNF expression in mouse hippocampal tissue, helping to assess the protective effects of hyperbaric oxygen on cognitive impairment.
Snippet: After blocking in normal goat serum for 30 min at room temperature, the waxed specimens were incubated with rabbit BDNF (Origene, USA;1:400) monoclonal antibody overnight at 4 °C. The sections were washed in 0.1 mol/L phosphate buffered saline (PBS) three ... Cite SaveSaving
Showing 1-6 of 6 papers.
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