EEF1A1 Rabbit Polyclonal Antibody

CAT#: TA326873

EEF1A1 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using EEF1A1 antibody.

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  Immunofluorescence analysis of HeLa cell using EEF1A1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:20- 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human EEF1A1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: eukaryotic translation elongation factor 1 alpha 1
• Database Link:
  NP_001393
  Entrez Gene 13627 MouseEntrez Gene 171361 RatEntrez Gene 1915 Human
  UniProt ID P68104
• Background: Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells . eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome. After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B. Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level.
• Synonyms: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EF-Tu; EF1A; GRAF-1EF; HNGC:16303; LENG7; PTI1

Reference Data

• Protein Families: Druggable Genome