BMI1 Rabbit Polyclonal Antibody

CAT#: TA325069

Rabbit polyclonal BMI1 Antibody

Product Images

Western blot analysis of BMI1 Antibody (Cat. #TA325069) in MCF7 cell line lysates (35ug/lane). BMI1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human breast carcinoma reacted with BMI1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of BMI1 Antibody (Cat#TA325069) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

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  BMI1 Antibody (Cat. #TA325069) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This BMI1 antibody is generated from rabbits immunized with BMI1 recombinant protein.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36949 Da
• Gene Name: BMI1 proto-oncogene, polycomb ring finger
• Database Link:
  NP_005171
  Entrez Gene 648 Human
  UniProt ID P35226
• Synonyms: BMI1; FLVI2; PCGF4; RNF51

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors