SPIN2B Rabbit Polyclonal Antibody

CAT#: TA323478

Anti-SPIN2B Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Hela cell lysate Primary antibody: TA323478 (SPIN2B Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA323478 (SPIN2B Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA323478 (SPIN2B Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela cell lysate
  IHC: 50-200
  Positive control: Human liver cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29 kDa
• Gene Name: spindlin family member 2B
• Database Link:
  NP_001006682
  Entrez Gene 474343 Human
  UniProt ID Q9BPZ2
• Background: Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal; but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary; SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also; SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells; SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M; which was more pronounced following IL3 withdrawal.
• Synonyms: dJ323P24.2; SPIN-2; SPIN-2B; SPIN2_duplicate; TDRD26