CDK1 Rabbit Polyclonal Antibody

CAT#: TA302015

Rabbit Polyclonal Antibody against CDC2 (N-term)

Product Images

Western blot analysis of CDC2 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC2 (arrow) was detected using the purified Pab.

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  Western blot analysis of CDC2 (arrow) using rabbit polyclonal CDC2 Antibody (N-term) (Cat.#TA302015).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2) (Origene Technologies).

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  Flow cytometric analysis of 293 cells using CDC2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: WB: 1:1000, FC: 1:10~50
• Reactivities: Human (Predicted: Bovine, Chicken)
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This CDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CDC2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34095 Da
• Gene Name: cyclin-dependent kinase 1
• Database Link:
  NP_001777
  Entrez Gene 983 Human
  UniProt ID P06493
• Background: CDC2 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
• Synonyms: CDC2; CDC28A; P34CDC2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Gap junction, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation