Human Lambda Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1402TR

Human Lambda Light Chain (F(ab)2 Fragment) goat polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human lambda light chain
• Specificity: This antibody detects Human Lambda Light chain.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
• Formulation: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer; 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer; 0.01% (w/v) Sodium Azide as preservative
  Label: Texas Red
  State: Lyophilized F(ab)2 Fragment
  Label: Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 1.6 moles Texas RedTM per mole of Goat IgG F(ab')2
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent). Dilute only prior to immediate use.
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation: Texas Red
• Storage: Store vial at 2-8 °C prior to restoration.
  For extended storage following reconstitution add glycerol to 50% and then aliquot contents and freeze at -20° C or below.
  Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 2-8 °C as an undiluted liquid.
• Stability: Shelf life: One year from despatch.