KLH Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | FLISA, IF, WB |
|---|---|
| Recommended Dilution | WB: User Optimized IF: 1:500-1:2,500 FLISA: User Optimized |
| Reactivity | KLH |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | KLH (Keyhole Limpet Hemocyanin |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, anti-Fluorescein and KLH. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Affinity chromatography |
| Conjugation | FITC |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Background | Anti-KLH antibody allows for the detection of KLH, important for monitoring immune Response, infection and inflammation. Keyhole limpet hemocyanin (KLH) is a large metal-containing protein consisting of 400 kDa subunits that is frequently used carrier protein in immunization procedures. KLH is most typically conjugated to a small molecule, peptide, or protein thereby rendering the complex highly immunogenic. KLH has been used as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy. This Anti-KLH antibody has been conjugated to fluorescein. |
| Synonyms | rabbit Anti-KLH antibody fluorescein conjugation, FITC conjugated rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin |
| Note | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Reference Data | |
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