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KLH Rabbit Polyclonal Antibody

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SKU
AP09215HR-N
Anti-KLH (RABBIT) Antibody Peroxidase Conjugated, 100 ug
Size:
100 ug
Conjugation:
HRP Unconjugated Unconjugated FITC Unconjugated Unconjugated
  $825.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:400,000
Reactivity KLH
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen KLH (Keyhole Limpet Hemocyanin
Specificity Anti-KLH (RABBIT) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, and KLH.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL - lot specific
Purification Immunoaffinity Chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation HRP
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Stability Expiration date is one (1) year from date of receipt.
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Background KLH Antibody, Peroxidase Conjugated, detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms rabbit Anti-KLH antibody peroxidase conjugation, HRP conjugated rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin
Note Anti-KLH Antibody Peroxidase Conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:60,000 is suggested for this product.  Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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