PHLPP (PHLPP1) (NM_194449) Human Recombinant Protein
SKU
TP303930
Recombinant protein of human PH domain and leucine rich repeat protein phosphatase (PHLPP), 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 184.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Cell treatment (PMID: 26971226) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_919431 |
| Locus ID | 23239 |
| UniProt ID | O60346 |
| Cytogenetics | 18q21.33 |
| RefSeq Size | 4798 |
| RefSeq ORF | 3615 |
| Synonyms | PHLPP; PLEKHE1; PPM3A; SCOP |
| Summary | This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. provided by RefSeq, Dec 2011 |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "PHLPP" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303930 | PHLPP1 MS Standard C13 and N15-labeled recombinant protein (NP_919431) | 10 ug |
$3,360.00
|
|
| LC405134 | Lyophilized PHLPP1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY405134 | Transient overexpression lysate of PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Discovery of a snail hibernation-inducer offering hibernation-like cardioprotection through metabolic rewiring and autophagy in mice
Piao, Zhang, Zhao et al
Nat Commun (2026)
Piao, Zhang, Zhao et al
Nat Commun (2026)
Cited by 1
AI Summary: OriGene’s human recombinant PHLPP1 protein (catalog number TP303930) was used in enzymatic assays to study the role of PHLPP1 in dephosphorylating AKT, helping elucidate the mechanism by which the snail-derived SNAP compound induces metabolic dormancy and cardioprotection.
Snippet: … The enzymatic reaction was carried out at 37°C for 2 hours in a reaction mixture containing 0.02 mg/ml BSA, 100 mM NaCl, 0.4 mM Mn²⁺, and 100 mM tricine (pH 7.4). For the reaction, 1.5 µM mouse fibroblast lysates, or 1.5 µM immunoprecipitated PHLPP1 from stressed mouse fibroblast lysates, or 1.5 µM human recombinant PHLPP1 (OriGene Technologies, TP303930) were utilized, along with 300 µM of the substrate. …
An inducer of snail hibernation causes quiescence and hibernation-like cardioprotection, through metabolic rewiring and autophagy, in mice hearts
Piao, Zhang, Zhao et al
bioRxiv (2026)
Piao, Zhang, Zhao et al
bioRxiv (2026)
AI Summary: OriGene’s human recombinant PHLPP1 protein (TP303930) was used in this study to assess the activity of the dormancy-inducing factor SNAP on PHLPP1-mediated signaling pathways, supporting the investigation of SNAP’s effects on metabolic rewiring and cardioprotection.
Snippet: For the reaction, 1.5 µM mouse 1000 fibroblast lysates, or 1.5 µM immunoprecipitated PHLPP1 from stressed mouse fibroblast lysates, or 1001 1.5 µM human recombinant PHLPP1 (OriGene Technologies, TP303930) were utilized, along with 1002 300 µM of the substrate
PHLPP1 deficiency ameliorates cardiomyocyte death and cardiac dysfunction through inhibiting Mcl-1 degradation
Tan, Li, Hu et al
Cell Signal (2022) 92, 110281 DOI: 10.1016/j.cellsig.2022.110281
Tan, Li, Hu et al
Cell Signal (2022) 92, 110281 DOI: 10.1016/j.cellsig.2022.110281
Cited by 18
AI Summary: OriGene’s recombinant PHLPP1 protein (TP303930) was used to perform dephosphorylation reactions in this study, enabling the authors to investigate the direct effect of PHLPP1 on Mcl-1 phosphorylation and degradation in cardiomyocytes.
Snippet: The dephosphorylation reactions were carried out with recombinant PHLPP1 protein (0.1 μg, OriGene Technologies, TP303930) and immunoprecipitated Mcl-1 protein at
Role of PHLPP1 in inflammation response: Its loss contributes to gliomas development and progression
Teng, Sun, An et al
Int. Immunopharmacol. (2016) 34, 229-234 DOI: 10.1016/j.intimp.2016.02.034
Teng, Sun, An et al
Int. Immunopharmacol. (2016) 34, 229-234 DOI: 10.1016/j.intimp.2016.02.034
Cited by 19
An ATP-site on-off switch that restricts phosphatase accessibility of Akt
Lin, Lin, Wu et al
Sci Signal (2012) 5 (223), ra37 DOI: 10.1126/scisignal.2002618
Lin, Lin, Wu et al
Sci Signal (2012) 5 (223), ra37 DOI: 10.1126/scisignal.2002618
Cited by 180
AI Summary: In this study, OriGene Technologies' recombinant full-length PHLPP1 was used to investigate how ATP-competitive inhibitors affect the accessibility of phosphorylated sites on Akt to phosphatases.
Snippet: His-Akt2 (Invitrogen) was incubated in the presence or absence of 10 μM GDC-0068 with recombinant full-length PHLPP1 (OriGene Technologies ... 8, 55–62 (1998).[CrossRef] [Web of Science][Medline];J. Yang, P. Cron, VM Good, V. Thompson, BA Hemmings, D. Barford, Crystal
Showing 1-5 of 5 papers.
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