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RNA Helicase A (DHX9) Human shRNA Plasmid Kit (Locus ID 1660)

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SKU
TG316515
DHX9 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
In Stock*

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Specifications
Specifications
Product Data
Locus ID 1660
Synonyms DDX9; LKP; NDH2; NDHII; RHA
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components DHX9 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 1660). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_001357, NM_030588, NR_033302, NM_001357.1, NM_001357.2, NM_001357.3, NM_001357.4, BC008773, BC014246, BC025245, BC032742, BC058896, BC071950, BC107881, BC137136, BC144494
UniProt ID Q08211
Summary This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.provided by RefSeq, Feb 2010
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

DHX9 helicase impacts on splicing decisions by modulating U2 snRNP recruitment in Ewing sarcoma cells
Frezza, Chellini, Riccioni et al
Nucleic Acids Res (2025) 53 (4) DOI: 10.1093/nar/gkaf068
Cited by 7
AI Summary: OriGene products pGFP-V-RS vector control (Cat# TR30013) and pGFP-V-RS_DHX9 shRNA vector (Cat# TG316515) were used to achieve DHX9 silencing in TC-71 cells, enabling the study of DHX9's role in alternative splicing in Ewing sarcoma.
Snippet: HX9 (Sigma Aldrich) at the final concentration of 25 nM. To obtain an additional DHX9 silencing, TC-71 cells were transfected with pGFP-V-RS vector control (shCTRL) or pGFP-V-RS_DHX9 vector (shDHX9) (Origene, Cat# TR30013 and Cat# TG316515, respectively), using Lipofectamine 2000 for 72 h. For the overexpression experiments, cells were transiently transfected with either GFP or GFP-DHX9, using Lip
FigureFigureFigure
The DNA/RNA helicase DHX9 orchestrates the KDM2B-mediated transcriptional regulation of YAP1 in Ewing sarcoma
Chellini, Scarfò, Bonvissuto et al
Oncogene (2023)
Cited by 8
AI Summary: OriGene products pGFP-V-RS vector control (Cat# TR30013) and pGFP-V-RS_DHX9 vector (Cat# TG316515) were used to generate stable DHX9-silenced and control TC-71 Ewing sarcoma cell lines, enabling the study of DHX9's role in tumor biology.
Snippet: … Resistance to chemotherapy and high propensity to metastasize remain the main causes Save;Stable clones were generated by transfecting TC-71 cells (DSMZ, Cat# ACC516) with pGFP-V-RS vector control (shCTRL) or pGFP-V-RS_DHX9 vector (Origene, Cat# TR30013 and Cat# TG316515, respectively), using Lipofectamine 2000 and by cell selection with 0,5 μg/ml Puromycin (MedChemExpres
Showing 1-2 of 2 papers.
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