GEF H1 (ARHGEF2) (NM_001162383) Human Tagged ORF Clone
- TrueORF®
SKU
RG229053
ARHGEF2 (tGFP-tagged) - Human Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), transcript variant 1
$1,554.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GEF H1 |
| Synonyms | GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM; P40 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001162383 |
| ORF Size | 2877 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001162383.1, NP_001155855.1 |
| RefSeq Size | 4194 bp |
| RefSeq ORF | 2961 bp |
| Locus ID | 9181 |
| UniProt ID | Q92974 |
| Cytogenetics | 1q22 |
| Protein Families | Druggable Genome |
| Protein Pathways | Pathogenic Escherichia coli infection |
| Summary | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.provided by RefSeq, Jun 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001162383" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC229053 | ARHGEF2 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), transcript variant 1 | 10 ug |
$1,354.00
|
|
| RC229053L3 | Lenti-ORF clone of ARHGEF2 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), transcript variant 1 | 10 ug |
$1,654.00
|
|
| RC229053L4 | Lenti-ORF clone of ARHGEF2 (mGFP-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), transcript variant 1 | 10 ug |
$1,654.00
|
|
| SC327688 | ARHGEF2 (untagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2) transcript variant 1 | 10 ug |
$1,355.00
|
|
Citations
Structural basis of microtubule-mediated signal transduction
Choi, Blum, Giono et al
Cell (2025)
Choi, Blum, Giono et al
Cell (2025)
Cited by 13
AI Summary: OriGene’s GEFH1, pCMV6-AC-GFP plasmid (Cat#RG229053) was used in this study to investigate the structural interaction between GEFH1 and microtubules, facilitating the elucidation of microtubule-mediated regulation of cellular signaling pathways.
Snippet: GEFH1, pCMV6-AC-GFP Origene Cat#RG229053 GEFH1, pFastBac-FLAG This study N/A GEFH1 Nter, pET28-6xHis This study N/A
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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