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SPNS2 (NM_001124758) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG225940
SPNS2 (tGFP-tagged) - Human spinster homolog 2 (Drosophila) (SPNS2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $710.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPNS2
Synonyms DFNB115; SLC62A2; SLC63A2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG225940 representing NM_001124758
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTGCCTGGAATGCGCCTCGGCGGCGGCGGGCGGCGCGGAGGAGGAGGAGGCGGACGCGGAGCGGC
GGCGCCGGCGCCGGGGGGCGCAGCGAGGGGCTGGCGGTAGCGGTTGCTGCGGGGCGCGGGGCGCGGGCGG
CGCTGGAGTCTCGGCCGCGGGCGATGAGGTGCAGACGCTGTCGGGCAGCGTAAGGCGGGCCCCGACCGGA
CCCCCCGGCACCCCCGGCACCCCCGGCTGCGCAGCTACTGCAAAGGGCCCCGGCGCTCAGCAGCCCAAAC
CGGCCAGCTTGGGCCGCGGGCGGGGGGCAGCCGCCGCCATCCTCAGCTTGGGCAACGTGCTCAACTACCT
GGACAGGTACACCGTGGCAGGCGTCCTTCTGGACATCCAGCAGCACTTTGGGGTCAAGGACCGAGGCGCC
GGCCTGCTGCAGTCAGTGTTCATCTGTAGCTTCATGGTGGCTGCCCCCATCTTCGGCTACCTGGGCGACC
GCTTCAACAGGAAGGTGATTCTCAGCTGCGGCATTTTCTTCTGGTCGGCCGTCACCTTCTCCAGCTCCTT
CATTCCCCAGCAGTACTTCTGGCTGCTGGTCCTGTCCCGGGGGCTGGTGGGCATCGGGGAGGCCAGCTAC
TCCACCATCGCCCCCACTATCATTGGCGACCTCTTCACCAAGAACACGCGTACGCTCATGCTGTCCGTCT
TCTACTTCGCCATCCCACTGGGCAGTGGCCTGGGCTACATTACTGGCTCCAGCGTGAAGCAGGCAGCCGG
AGACTGGCACTGGGCATTGCGGGTGTCCCCTGTCCTGGGCATGATCACAGGAACACTCATCCTCATTCTG
GTCCCAGCCACTAAAAGGGGTCATGCCGACCAGCTCGGGGACCAGCTCAAGGCCCGGACCTCATGGCTCC
GAGATATGAAGGCCCTGATTCGAAACCGCAGCTACGTCTTCTCCTCCCTGGCCACGTCGGCTGTCTCCTT
CGCCACGGGGGCCCTGGGCATGTGGATCCCGCTCTACCTGCACCGCGCCCAAGTTGTGCAGAAGACAGCA
GAGACGTGCAACAGCCCGCCCTGTGGGGCCAAGGACAGCCTCATCTTTGGGGCCATCACCTGCTTTACGG
GATTTCTGGGCGTGGTCACGGGGGCAGGAGCCACGCGCTGGTGCCGCCTGAAGACCCAGCGGGCCGACCC
ACTGGTGTGTGCCGTGGGCATGCTGGGCTCTGCCATCTTCATCTGCCTGATCTTCGTGGCTGCCAAGAGC
AGCATCGTAGGAGCCTATATCTGTATCTTCGTCGGGGAGACGCTGCTGTTTTCTAACTGGGCCATCACTG
CAGACATCCTCATGTACGTGGTCATCCCCACGCGGCGCGCCACTGCCGTGGCCTTGCAGAGCTTCACCTC
CCACCTGCTGGGGGACGCCGGGAGCCCCTACCTCATTGGCTTTATCTCAGACCTGATCCGCCAGAGCACT
AAGGACTCCCCGCTCTGGGAGTTCCTGAGCCTGGGCTACGCGCTCATGCTCTGCCCTTTCGTCGTGGTCC
TGGGCGGCATGTTCTTCCTCGCCACTGCGCTCTTCTTCGTCAGCGACCGCGCCAGGGCTGAGCAGCAGGT
GAACCAGCTGGCGATGCCGCCCGCATCTGTGAAAGTC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG225940 representing NM_001124758
Red=Cloning site Green=Tags(s)

MMCLECASAAAGGAEEEEADAERRRRRRGAQRGAGGSGCCGARGAGGAGVSAAGDEVQTLSGSVRRAPTG
PPGTPGTPGCAATAKGPGAQQPKPASLGRGRGAAAAILSLGNVLNYLDRYTVAGVLLDIQQHFGVKDRGA
GLLQSVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASY
STIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTLILIL
VPATKRGHADQLGDQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWIPLYLHRAQVVQKTA
ETCNSPPCGAKDSLIFGAITCFTGFLGVVTGAGATRWCRLKTQRADPLVCAVGMLGSAIFICLIFVAAKS
SIVGAYICIFVGETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIRQST
KDSPLWEFLSLGYALMLCPFVVVLGGMFFLATALFFVSDRARAEQQVNQLAMPPASVKV

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001124758
ORF Size 1647 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001124758.3
RefSeq Size 4349 bp
RefSeq ORF 1650 bp
Locus ID 124976
UniProt ID Q8IVW8
Cytogenetics 17p13.2
Protein Families Transmembrane
Summary The protein encoded by this gene is a transporter of sphingosine 1-phosphate, a secreted lipid that is important in cardiovascular, immunological, and neural development. Defects in this gene are a cause of early onset progressive hearing loss. provided by RefSeq, Jul 2016
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Documents
Resources
"NM_001124758" in other vectors (6)
SKU Description Size Price
RC225940 SPNS2 (Myc-DDK-tagged)-Human spinster homolog 2 (Drosophila) (SPNS2) 10 ug
$289.00 $510.00
RC225940L1 Lenti-ORF clone of SPNS2 (Myc-DDK-tagged)-Human spinster homolog 2 (Drosophila) (SPNS2) 10 ug
$810.00
RC225940L2 Lenti-ORF clone of SPNS2 (mGFP-tagged)-Human spinster homolog 2 (Drosophila) (SPNS2) 10 ug
$810.00
RC225940L3 Lenti-ORF clone of SPNS2 (Myc-DDK-tagged)-Human spinster homolog 2 (Drosophila) (SPNS2) 10 ug
$810.00
RC225940L4 Lenti-ORF clone of SPNS2 (mGFP-tagged)-Human spinster homolog 2 (Drosophila) (SPNS2) 10 ug
$810.00
SC323131 SPNS2 (untagged)-Human spinster homolog 2 (Drosophila) (SPNS2) 10 ug
$512.00

Plasmid Map for RG225940

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG225940
Circular map for RG225940

file-alt Citations

SPNS2 exports sphingosine-1-phosphate and imports glucose
Weigel, Hossen, Brown et al
Nat Commun (2026) 17 (1) DOI: 10.1038/s41467-026-71659-7
Cited by 2
AI Summary: In this study, OriGene’s SPNS2 (NM_001124758) Human Tagged ORF Clone in pCMV6-AC-GFP and SPNS2 (NM_153060) Mouse Tagged ORF Clone in pCMV6-AC-G were used to express SPNS2 proteins, enabling the investigation of their roles in exporting sphingosine-1-phosphate and importing glucose.
Snippet: SPNS2 (NM_001124758) Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene), 2 µg of Spns2 (NM_153060) Mouse Tagged ORF Clone in pCMV6-AC-G
FigureFigureFigure
Showing 1-1 of 1 papers.
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