pLenti-P2A-Puro Lentiviral Gene Expression Vector with EF1a promoter

SKU
PS100142
pLenti-P2A-Puro Lentiviral Gene Expression Vector with EF1a promoter, 10ug
  $880.00
In Stock*
Specifications
Specifications
Product Data
E. coli Selection Chloramphenicol
Mammalian Cell Selection Puromycin
Expression Promoter EF1a
Features
  • This Lenti vector is a third generation lentiviral vector.
  • All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells
  • EF51 Primer can be used to sequence the target sequence after cloning
  • P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
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Documents
Resources

file-alt Citations

Pegargiminase Suppresses the Fanconi Anemia Pathway and Promotes Melphalan-Induced DNA Double-Strand Breaks in Uveal Melanoma
Pavlyk, Field, Young et al
Pigment Cell Melanoma Res (2026) 39 (4), e70104 DOI: 10.1111/pcmr.70104
AI Summary: In this study, OriGene products TurboFectin 8.0 (a transfection reagent) and the Lenti-vpak packaging system were used to facilitate gene delivery and transfection experiments in uveal melanoma cell lines, supporting the investigation of pegargiminase and melphalan effects.
Snippet: , #PS100142), together with the Lenti- vpak packaging system (OriGene, #TR30037). Transfection was performed using TurboFectin 8.0 (OriGene) in a 6- well plate format
FigureFigureFigure
Epigenetic control of microglial mitochondrial immunity by KAT7 drives Alzheimer's disease pathogenesis
Liu, Ye, Fan et al
Neuron (2026)
Cited by 1
AI Summary: OriGene’s pLenti-EF1a-P2A-Puro vector (Cat #PS100142) was used in this study to generate lentiviral constructs for expressing genes of interest, enabling the investigation of KAT7’s role in microglial mitochondrial immunity and Alzheimer’s disease pathogenesis.
Snippet: This paper N/A Recombinant DNA pLentiCRISPR-v2-Puro vector Sanjana et al.59 RRID: Addgene_52961 pLentiCRISPR-Kat7-sgRNA This paper N/A pLenti-EF1a-P2A-Puro vector Origene Cat #PS100142 [https://www.ncbi.nlm.nih.gov/nuccore/PS100142] pLenti-EF1a-KAT7 This paper N/A pLenti-EF1a-KAT7 (E508Q) This paper N/A pLenti-EF1a-JADE2 This pape
Showing 1-2 of 2 papers.
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