pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

SKU
PS100093
pLenti-C-mGFP-P2A-Puro, Lenti vector with C-terminal mGFP tag and P2A-Puro, 10 ug
  $880.00
In Stock*
Specifications
Specifications
Product Data
C-Tag mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal monomeric GFP tag.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • mGFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

FBXO28 targets SNAI1 for ubiquitin-proteasomal degradation in non-small cell lung cancer
Lin, Qiao, Liu et al
J Biol Chem (2026)
AI Summary: OriGene products—including the pCMV6-FBXO28-Myc-Flag, pLenti-C-mGFP-P2A, pRS-shSNAI1, pRS-shSNAI2, and pRS-shRNA control plasmids—were used in this study to manipulate gene expression and investigate the role of FBXO28 in regulating SNAI1 stability and metastasis in non-small cell lung cancer.
Snippet: pCMV6-FBXO28-Myc-Flag (#RC206490), pLenti-C-mGFP-P2A (#PS100093), pRS-shSNAI1 (#TR309226A), pRS-shSNAI2 (#TR309225B) and pRS-shRNA control (#TR30012) plasmids were acquired from Origene
Intracellular SPP1 inhibits FNDC5 to activate DEGS1-regulated ceramide metabolism in silicosis
Li, Jin, Li et al
Journal of Pharmaceutical Analysis (2026)
AI Summary: OriGene products contributed to this study by providing control plasmids (PS100093) and Spp1 overexpression plasmids (MR226579L4), which were used to manipulate gene expression in cell transfection experiments investigating the molecular mechanisms of silicosis.
Snippet: Cells were transfected with the following constructs, as specified in individual experiments: control plasmids (PS100093, OriGene Technologies Inc., Rockville, MD, USA), Spp1 overexpression plasmids (MR226579L4, OriGene Technologies Inc., Rockville,
Intratumoral microbiota and host genotype cooperatively shape neutrophil cytotoxic functions in colorectal cancer
Sorrenti, Governa, Bressan et al
Cell Host Microbe (2026) 34 (3), 425-443.e11 DOI: 10.1016/j.chom.2026.02.006
Cited by 8
AI Summary: OriGene products, including the pLenti-C-mGFP-P2A-Puro vector (Cat#PS100093), Human SIGLEC5 ORF clone (mGFP-tagged, Cat#RC206610L4), and Human SIGLEC14 ORF clone (mGFP-tagged, Cat#RC224202L4), were used to investigate the roles of Siglec-5 and Siglec-14 in neutrophil responses to intratumoral microbiota in colorectal cancer.
Snippet: pLenti-C-mGFP-P2A-Puro Origene Cat#PS100093 Human SIGLEC5 ORF clone (mGFP-tagged) Origene Cat#RC206610L4 Human SIGLEC14 ORF clone (mGFP-tagged) Origene Cat#RC224202L4
Targeting the STK39/ARID2 Axis to Inhibit NF-κB Signaling: A Novel Pathway for Mesenchymal Stem Cell Osteogenic Differentiation in Osteoporosis Management
Wang, Wei, Chen et al
J Musculoskelet Neuronal Interact (2026) 26 (1), 124-133 DOI: 10.22540/JMNI-26-124
AI Summary: OriGene products, specifically the negative control siRNA (siNC; TR30023) and negative control plasmid (NC; PS100093), were used as experimental controls in gene silencing and overexpression studies to investigate the roles of ARID2 and STK39 in mesenchymal stem cell differentiation in osteoporosis research.
Snippet: ding negative controls (siNC;TR30023 and NC;PS100093), were obtained from OriGene (Rockville, MD, USA). BMSCs were allocated to the following groups: siNC, siARID2, NC + siNC, NC + siARID2, STK39 overexpression + siNC, and STK39 overexpression + siARID2. For transduction, 1
FigureFigureFigure
Renal cancer cell-derived amphiregulin recruits mesenchymal stromal cells, induces their glycolytic switch, and promotes tumour growth
Popławski, Grzanka, Skirecki et al
bioRxiv (2025)
AI Summary: OriGene’s pLenti-C-mGFP-P2A-Puro_AREG, pLenti-C-mGFP-P2A-Puro_FN1, and pLenti-C-mGFP-P2A-Puro vectors were used to manipulate gene expression in renal cancer and mesenchymal stromal cells, enabling the study of amphiregulin’s role in MSC recruitment, metabolic reprogramming, and tumour growth.
Snippet: Vectors pLenti-C-mGFP-P2A-Puro_AREG (Origene CAT#: RC203150L4), pLenti-C-mGFP-P2A-Puro_FN1 (Origene CAT#: RC212860L4), and empty vector pLenti-C-mGFP-P2A-Puro (CAT#: PS100093) were purchased from Origene (Rockville, MD)
Modulating the serine metabolism in human differentiated astrocytes: an integrated multi omics approach
Tripodi, Maffioli, Sacchi et al
Front Cell Neurosci (2025) 19, 1616911 DOI: 10.3389/fncel.2025.1616911
Cited by 2
AI Summary: OriGene contributed lentiviral vectors (RC203949L4 for PHGDH, RC202475L4 for PSAT, RC209090L4 for PSP, and PS100093 as control) that enabled the overexpression of GFP-tagged phosphorylated pathway enzymes in human astrocytes, facilitating the study of serine metabolism modulation in this research.
Snippet: Lentiviral vectors to obtain overexpression of GFP-tagged PHGDH, PSAT and PSP (together with the empty GFP vector) under a CMV promoter were purchased from Origene (Catalog numbers RC203949L4 for PHGDH, RC202475L4 for PSAT, RC209090L4 for PSP and PS100093 as control)
FigureFigureFigure
Showing 1-6 of 38 papers.
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