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pLenti-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector

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SKU
PS100092
pLenti-C-Myc-DDK-P2A-Puro, Lenti vector with C-terminal Myc-DDK tag and P2A-Puro, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with C-terminal Myc-DDK tags.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Unc-51 like kinase 3 (ULK3) contributes to autophagy and cell survival in multiple myeloma
Tauro, Li, Sudalagunta et al
Nature Communications (2026)
AI Summary: OriGene’s pLenti-C-MycDDK-P2A-Puro vector (CAS PS100092) was used to transduce ULK3-knockdown multiple myeloma cells with ULK3 or control constructs, enabling functional studies of ULK3’s role in autophagy and cell survival.
Snippet: ULK3-knockdown U266 cells were transduced with lentiviral particles encoding ULK3 or a non-targeting control using the pLenti-C-MycDDK-P2A-Puro vector (Origene, CAS PS100092) (Fig. 3). Cells were seeded in antibiotic-free RPMI and transduced at MOI 5 or 10 in the presence of Vectofusin-1. …
Pancreatic Adenocarcinoma Up-Regulated Factor Promotes Epithelial-Mesenchymal Transition and Lung Metastasis in Hepatocellular Carcinoma
Park, Ham, Lee et al
Int J Mol Sci (2026) 27 (14) DOI: 10.3390/ijms27146213
AI Summary: OriGene products, including TGF-βRI and TGF-βRII siRNA oligo duplexes, as well as the pCMV6-XL5, pCMV-TGF-βRI, pCMV-TGF-βRII, pLenti-control, pLenti-PAUF, and pLenti-Luc vectors, were used in this study to manipulate gene expression and investigate the role of PAUF in epithelial-mesenchymal transition and lung metastasis in hepatocellular carcinoma cells.
Snippet: … TGF-βRI (cat# SR322023) and TGF-βRII (cat# SR322024) siRNA oligo duplexes were obtained from OriGene Technologies, Inc. … pCMV6-XL5 (cat# PCMV6XL5), pCMV-TGF-βRI (cat# SC108520), pCMV-TGF-βRII (cat# SC302306), pLenti-control (cat# PS100092), and pLenti-PAUF (cat# RC202244L3), and pLenti-Luc (cat# PS100138) vectors were obtained from OriGene Technologies, Inc
FigureFigureFigure
Discovery of BEND4 as a novel single-gene prognostic marker and therapeutic target for adverse AML
Banerjee, Mishra, Dsouza et al
NPJ Precis Oncol (2026)
AI Summary: OriGene products pLenti-c-Myc-DDK-P2A-Puro-BEND4 (CAT: RC212072L3) and pLenti-C-Myc-DDK-P2A-Puro (CAT: PS100092) were used to achieve BEND4 overexpression and serve as vector controls, enabling the study of BEND4’s role as a prognostic marker and therapeutic target in AML.
Snippet: … Cytogenetic and mutation-based;For BEND4 overexpression, pLenti - c -Myc -DDK -P2A -Puro -BEND4 (Origene, CAT: RC212072L3) was used, and pLenti - c -MYC -DDK -Puro -GFP (Addgene, 123299) or pLenti - C -Myc -DDK -P2A - Puro (Origene, CAT: PS100092) were used as vector control
SOX9 and SEMA7A regulate cell plasticity in the postpartum mammary gland with implications for breast cancer
Cozzens, Hinckley, Elder et al
bioRxiv (2026)
AI Summary: OriGene products contributed to this study by providing the SEMA7A-ORF Clone plasmid (#MR209876L3) for generating SEMA7A overexpressing cell lines and the empty vector control plasmid (#PS100092), enabling functional analysis of SEMA7A’s role in mammary epithelial cell plasticity.
Snippet: To 573 generate SEMA7A OE cell lines, plasmids containing a SEMA7A-ORF Clone insert (Origene, #MR209876L3, 574 pLenti-C-Myc-DDK-P2A-puro backbone) were packaged into lentivirus at the CU Functional Genomics Shared 575 Resource. The same backbone without an ORF insert was used as an empty vector control (Origene, 576 #PS100092)
In Vitro characterization of narciclasine: solubility, metabolic stability, and P-glycoprotein substrate status
Akkireddy, Yang, Lenkalapelly et al
Frontiers in Pharmacology (2026) 17 DOI: 10.3389/fphar.2026.1768477
Cited by 2
AI Summary: OriGene products contributed to this study by providing the lentiviral ABCB1 plasmid (RC216080L3), the control plasmid (PS100092), and the Lenti-vpak Packaging kit, which were used to generate human cells overexpressing P-glycoprotein for evaluating narciclasine’s substrate status.
Snippet: HEK-293FT cells were plated and incubated until 70% confluent. The cells were then co-transfected with either the lentiviral ABCB1 (RC216080L3) plasmid or the control (PS100092) plasmid, along with the Lenti-vpak Packaging kit (OriGene)
Resistance to neoadjuvant talazoparib in triple-negative breast cancer by BRN2-induced ATR/STAT3 pathways or SHLD2 subclone expansion
Abdulkareem, Jiang, Qi et al
Proc Natl Acad Sci U S A (2026) 123 (16), e2513742123 DOI: 10.1073/pnas.2513742123
Cited by 1
AI Summary: OriGene products, including lentiviruses encoding human BRN2 (Cat. No. RC205330) and BRN2 shRNAs (Cat. No. TRCN0000019329 and TRCN0000019332), were used in this study to manipulate BRN2 expression and investigate its role in PARP inhibitor resistance in triple-negative breast cancer.
Snippet: mlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="pmc:entrez-nucleotide" xlink:href="PS100092">PS100092) and lentiviruses encoding human BRN2 (Cat. No. RC205330) were purchased from OriGene. For knockdown experiments, lentivirus containing BRN2 shRNAs (Cat. No. TRCN0000019329, shRNA #1;and Cat. No. TRCN0000019332, shRNA #2) and a nontargeting control (Cat. No. SHC007) were obtai
Showing 1-6 of 51 papers.
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