Sp7 (NM_130458) Mouse Tagged Lenti ORF Clone
- LentiORF®
SKU
MR226379L4
Lenti ORF clone of Sp7 (mGFP-tagged) - Mouse Sp7 transcription factor 7 (Sp7)
$986.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Sp7 |
| Synonyms | 6430578P22Rik; C22; Osx |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_130458 |
| ORF Size | 1284 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_130458.3 |
| RefSeq Size | 2284 bp |
| RefSeq ORF | 1287 bp |
| Locus ID | 170574 |
| UniProt ID | Q8VI67 |
| Cytogenetics | 15 F3 |
| Summary | Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_130458" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC212316 | Sp7 (untagged) - Mouse Sp7 transcription factor 7 (Sp7), (10ug) | 10 ug |
$457.00
|
|
| MG226379 | Sp7 (tGFP-tagged) - Mouse Sp7 transcription factor 7 (Sp7), (10ug) | 10 ug |
$886.00
|
|
| MR226379 | Sp7 (Myc-DDK-tagged) - Mouse Sp7 transcription factor 7 (Sp7) | 10 ug |
$686.00
|
|
| MR226379L1 | Lenti ORF clone of Sp7 (Myc-DDK-tagged) - Mouse Sp7 transcription factor 7 (Sp7) | 10 ug |
$986.00
|
|
| MR226379L2 | Lenti ORF clone of Sp7 (mGFP-tagged) - Mouse Sp7 transcription factor 7 (Sp7) | 10 ug |
$986.00
|
|
| MR226379L3 | Lenti ORF clone of Sp7 (Myc-DDK-tagged) - Mouse Sp7 transcription factor 7 (Sp7) | 10 ug |
$986.00
|
|
Citations
Bone-derived Osterix+ osteolineage cells are a source of tumor-promoting myofibroblastic cancer-associated fibroblasts in breast cancer
Furesi, Zeng, Eul et al
Nat Commun (2026)
Furesi, Zeng, Eul et al
Nat Commun (2026)
AI Summary: OriGene’s pLenti-puro ORF clone of Sp7 (mGFP-tagged, OriGene MR226379L4) was used in this study to ectopically express Sp7 (Osx) in mouse mammary fibroblasts and immortalized cancer-associated fibroblasts, enabling investigation of Osx’s role in CAF differentiation and tumor-promoting activity.
Snippet: The puromycin -resistant pLenti -puro ORF clone of Sp7 (mGFP -tagged Origene MR226379L4) was used to ectopically express Sp7 (Osx) in MMFs or immortalized CAFs. pLenti -puro (Addgene plasmid #39481) was used as a vehicle control
Myofibroblastic CAFs arising from bone-resident osteoblast precursors retain an osteolineage signature and support breast cancer progression via Osterix-mediated signaling
Furesi, Zeng, Eul et al
bioRxiv (2025)
Furesi, Zeng, Eul et al
bioRxiv (2025)
AI Summary: OriGene’s mGFP-tagged Sp7 plasmid (MR226379L4) was used in this study to ectopically express Sp7 (Osterix) in myofibroblastic cancer-associated fibroblasts (CAFs) and immortalized CAFs, enabling the investigation of Osterix-mediated signaling in breast cancer progression.
Snippet: e of Sp7 (mGFP-tagged – Origene MR226379L4) was used to ectopically express Sp7 (Osx) in MMFs or immortalized CAFs. …
Showing 1-2 of 2 papers.
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