Hmgcs2 (BC014714) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG208162
Hmgcs2 (tGFP-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (cDNA clone MGC:25633 IMAGE:4216637)
$911.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hmgcs2 |
| Synonyms | mHS, 1300002P16 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC014714 |
| ORF Size | 1524 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC014714, AAH14714 |
| RefSeq Size | 2011 bp |
| RefSeq ORF | 1526 bp |
| Locus ID | 15360 |
| Cytogenetics | 3 42.74 cM |
| Summary | This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Ascites protects against ferroptosis and enables the peritoneal growth of ovarian cancer
Setayeshpour, Chen, Dayanidhi et al
Nat Commun (2026) 17 (1) DOI: 10.1038/s41467-026-72116-1
Setayeshpour, Chen, Dayanidhi et al
Nat Commun (2026) 17 (1) DOI: 10.1038/s41467-026-72116-1
Cited by 4
AI Summary: OriGene products contributed to this study by providing the mouse HMGCS2 ORF clone in the pCMV6-Entry backbone (MG208162 and RC208128) for overexpression experiments and the lentiviral vector (PS100069) for generating stable cell lines, enabling functional analyses of HMGCS2 in ovarian cancer ferroptosis resistance.
Snippet: … For transient overexpression of HMGCS2, 100 ng of mouse _hmgcs2_ ORF clone with a pCMV6-Entry backbone (Origene, MG208162) was diluted in Opti-MEM I (9 µL). 0.3 µL of the TransIT -LT1 transfection reagent (Mirus, MIR2305) was added before the mix was incubated for 15 min in a 96-well plate;plasmids was conducted using the Qiagen Plasmid Midi kit (12145). For transient overexpression of HMGCS2, 100 ng of mouse hmgcs2 ORF clone with a pCMV6-Entry backbone (Origene, MG208162) was diluted in Opti-MEM I (9 µL). 0.3 µL of the;- HMGCS2 ORF clone with a pCMV6-Entry backbone (Origene, RC208128) was cut and ligated into a lentiviral vector (Origene, PS100069) according to the manufacturer’s
Ascitic fluid protects against ferroptosis and enables the peritoneal spread of ovarian cancer
Setayeshpour, Chen, Dayanidhi et al
bioRxiv (2024)
Setayeshpour, Chen, Dayanidhi et al
bioRxiv (2024)
Cited by 2
Showing 1-2 of 2 papers.
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in shipping.
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