Mito Glow

Mito-Glow Lentiviral EF1a-MLS-mScarlet Human Lentiviral Particle

Lentiviral particles encoding CMV-driven TOMM20-mGFP for stable green fluorescent labeling of the mitochondrial outer membrane in living cells. The vector encodes puromycin resistance for selection of transduced cells. mGFP (TagGFP2) • green fluorescence • Ex 483 nm / Em 506 nm; pKa 4.7; maturation ~11 min; monomeric; 26.9 kDa

HeLa cells were transduced with Mito-Glow EF 1a-TOMM20-mGFP lentiviral particles at an MOI of 4 and imaged following 10 days of puromycin selection. Cells were stained with 100 nM MitoTracker™ Red and 1 g/mL Hoechst 33342 and imaged live using a Keyence fluorescence microscope with a 40x objective. GFP fluorescence confirms reporter expression following transduction, while overlap with MitoTracker Red supports mitochondrial localization. Hoechst 33342 labels nuclei.

HeLa cells were transduced with Mito-Glow CMV-MLS-mGFP lentiviral particles at an MOI of 4 and imaged following 10 days of puromycin selection. Cells were stained with 100 nM MitoTracker™ Red and 1 g/mL Hoechst 33342 and imaged live using a Keyence fluorescence microscope with a 40x objective. GFP fluorescence confirms reporter expression following transduction, while overlap with MitoTracker Red supports mitochondrial localization. Hoechst 33342 labels nuclei.

HeLa cells were transduced with Mito-Glow EF1a-MLS-mGFP lentiviral particles at an MOI of 4 and imaged following 10 days of puromycin selection. Cells were stained with 100 nM MitoTracker™ Red and 1 ug/mL Hoechst 33342 and imaged live using a Keyence fluorescence microscope with a 40x objective. GFP fluorescence confirms reporter expression following transduction, while overlap with MitoTracker Red supports mitochondrial localization. Hoechst 33342 labels nuclei.

HeLa cells were transduced with Mito-Glow CMV-MLS-mScarlet3 lentiviral particles at an MOI of 4 and imaged following 10 days of puromycin selection. Cells were stained with 100 nM MitoTracker™ Green and 1 ug/mL Hoechst 33342 and imaged live using a Keyence fluorescence microscope with a 60x objective. mScarlet3 fluorescence confirms reporter expression following transduction, while overlap with MitoTracker Green supports mitochondrial localization. Hoechst 33342 labels nuclei.

HeLa cells stably expressing CMV-driven TOMM20-mGFP were stained with 100 nM MitoTracker™ Red and 1 g/mL Hoechst 33342 and imaged live using a Keyence fluorescence microscope with a 60* objective. Overlap between the GFP and MitoTracker Red signals supports mitochondrial localization of the reporter. Hoechst 33342 labels nuclei

HeLa cells stably expressing CMV-driven MLS-mGFP were stained with 100 nM MitoTracker™ Red and 1 g/mL Hoechst 33342 and imaged live using a Keyence fluorescence microscope with a 40x objective. Overlap between the GFP and MitoTracker Red signals supports mitochondrial localization of the reporter. Hoechst 33342 labels nuclei.

HeLa cells stably expressing CMV-driven MLS-mScarlet3 were stained with 100 nM MitoTracker™ Green and 1 g/mL Hoechst 33342 and imaged live using a Keyence fluorescence microscope with a 60x objective. Overlap between the mScarlet3 and MitoTracker Green signals supports mitochondrial localization of the reporter. Hoechst 33342 labels nuclei.

Mito-Glow Lentiviral hSyn-MLS-mGFP Human Lentiviral Particle