EV Purity

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOE (RC200395, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOE. Positive lysates LY424959 (100ug) and LC424959 (20ug) can be purchased separately from OriGene.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody TA336279 - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (TA336279) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.