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pRFP-C-shLenti shRNA Vector

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SKU
TR30030
HuSH shRNA RFP Lenti Cloning Vector (pRFP-C-shLenti Vector), 5 ug
  $880.00
In Stock*
Specifications
Specifications
Product Data
Control Type Negative
Fluorescent Label None
Mammalian Cell Selection Puromycin
Viral Packaging Lentiviral
Features
  • pRFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tRFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
  • U6_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Components 1 vial of 5 ug lyophilized pRFP-C-shLenti shRNA cloning vector
Quality Control This vector was sequence verified and functionally validated.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
Resources

file-alt Citations

Myeloid cell-specific β2-adrenergic receptor deletion improves early cardiac injury resolution via de-repression of Anxa1
Nayak, Bajpai, Patwa et al
Theranostics (2026) 16 (8), 3959-3976 DOI: 10.7150/thno.123651
Cited by 3
AI Summary: In this study, OriGene’s TR30030 pRFP-C-shLenti vector was used to deliver shRNA targeting Annexin A1 (Anxa1) into bone marrow cells, enabling researchers to investigate the role of Anxa1 in myeloid cell-mediated cardiac injury resolution.
Snippet: one marrow transplant (BMT)Bone marrow cells from the femurs of LB2 mice were isolated and transduced with either scrambled or Annexin A1 (Anxa1) shRNA using TR30030 pRFP-C-shLenti vector (Origene Technologies Inc. MD, USA) at an MOI of 150 (Table S2). Lentivirus transduction was performed in serum free RPMI-1640 (w/o antibiotic and antimycotic) in
FigureFigureFigure
Loss of Myeloid Cell-Specific β2-Adrenergic Receptor Expression Ameliorates Cardiac Function and Remodeling after Acute Ischemia
Nayak, Bajpai, Patwa et al
bioRxiv (2023)
Cited by 1
Snippet: Bone marrow cells from the femurs of LB2 mice were isolated and transduced with either scrambled or Annexin A1 (Anxa1) shRNA using TR30030 pRFP-C-shLenti vector (Origene Technologies Inc. MD, USA) at an MOI of 150. Lentivirus transduction was performed in serum free RPMI-1460 (w/o antibiotic and antimycotic) in the presence of ViralEntry Transduction Enhancer (Applied Biological Materials Inc. …
Transcription factors TEAD2 and E2A globally repress acetyl-CoA synthesis to promote tumorigenesis
Park, Mossmann, Chen et al
Mol Cell (2022) 82 (22), 4246-4261.e11 DOI: 10.1016/j.molcel.2022.10.027
Cited by 31
AI Summary: In this study, OriGene products—specifically the TEAD2 (Cat# SR307879) and E2A (Cat# SR305024) shRNA lentiviral particles—were used to knock down TEAD2 and E2A expression, enabling the authors to demonstrate that these transcription factors repress acetyl-CoA synthesis and thereby promote tumorigenesis.
Snippet: Transcription factors TEAD2 and E2A globally repress acetyl-CoA synthesis to promote tumorigenesis
Showing 1-3 of 3 papers.
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