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pB-RS shRNA Vector

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SKU
TR30024
HuSH shRNA Retroviral Cloning Vector with blasticidin resistance (pB-RS Vector), 5 ug
  $470.00
In Stock*

Frequently Bought Together (1)

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5 x 200 ul
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Specifications
Specifications
Product Data
Control Type Negative
Fluorescent Label None
Mammalian Cell Selection Blasticidin
Viral Packaging Retroviral
Features
  • The HuSH pB-RS plasmid vector incorporates all the elements as the pRS vector with blasticidin in mammalian selection instead of puromycin.
  • U6_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Components 1 vial of 5 ug lyophilized pB-RS shRNA cloning vector
Quality Control This vector was sequence verified and functionally validated.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Effects of Wood Smoke Constituents on Mucin Gene Expression in Mice and Human Airway Epithelial Cells and on Nasal Epithelia of Subjects with a Susceptibility Gene Variant in Tp53
Tassew, Fort, Mebratu et al
Environ Health Perspect (2022) 130 (1), 17010 DOI: 10.1289/EHP9446
AI Summary: In this study, OriGene Technologies’ SLC26A9-specific shRNA plasmid (Cat. no. TR301620) and control shRNA plasmid (Cat. no. TR30024) were used to silence SLC26A9 expression in HEK293T cells, enabling the researchers to investigate the role of this protein in wood smoke-induced mucin gene expression.
Snippet: Silencing Using Short Hairpin RNA Retroviral silencing vector encoding for SLC26A9 short hairpin RNA (shRNA) and control (Ctrl) vector were used. SLC26A9-specific shRNA plasmids and control plasmids (OriGene Technologies;Cat. nos. TR301620 and TR30024) were directly used in transfected HEK293T cells using Mirus 2020 transfection reagent and packaged into retroviral particles using Phoenix cells (
FigureFigureFigure
Numb-like (NumbL) downregulation increases tumorigenicity, cancer stem cell-like properties and resistance to chemotherapy
García-Heredia, Verdugo Sivianes, Lucena-Cacace et al
Oncotarget (2016) 7 (39), 63611-63628 DOI: 10.18632/oncotarget.11553
Cited by 48
AI Summary: OriGene contributed shRNA constructs targeting NumbL, Numb, and a scrambled sequence control (pB-RS vectors) used to downregulate gene expression and investigate the role of NumbL in tumorigenicity, cancer stem cell properties, and chemoresistance.
Snippet: To downregulate NumbL expression, short hairpins RNA (shRNA) against NumbL, Numb or scrambled sequence control in pB-RS vectors were obtained from Origene (Rockville, MD)
FigureFigureFigure
Modification of cytokine-induced killer cells with chimeric antigen receptors (CARs) enhances antitumor immunity to epidermal growth factor receptor (EGFR)-positive malignancies
Ren, Ma, Lu et al
Cancer Immunol. Immunother. (2015)
Cited by 26
AI Summary: OriGene’s 29mer shRNA constructs in a retroviral vector were used to generate stable EGFR-knockdown cell lines (A549, SGC7901, and HT29), enabling the study to assess the specificity and efficacy of CAR-modified cytokine-induced killer cells against EGFR-positive malignancies.
Snippet: The stable EGFR- knockdown cells of A549, SGC7901, and HT29 were gen- erated by transfecting the cells with the 29mer shRNA con- structs in a retroviral vector (OriGene Technologies, Inc., Rockville, MD, USA) using jetPEI transfection reagent (PolyPlus Transfection ... Cite SaveSaving
Showing 1-3 of 3 papers.
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