pGFP-V-RS shRNA Vector

CAT#: TR30007

HuSH shRNA GFP Retroviral Cloning Vector (pGFP-V-RS Vector), 5 ug

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Specifications

Product Data

• Control Type: Negative
• Fluorescent Label: GFP
• Viral Packaging: Retroviral
• Feature:
  • The HuSH pGFP-V-RS plasmid vector contains both 5' and 3' LTRs of Moloney murine leukemia virus (MMLV) that flank the puromycin marker and the U6-shRNA expression cassette. Upon transient transfection of the plasmids into a packaging cell line, replication deficient viruses can be obtained and used to infect target cells. The puromycin-N-acetyl transferase gene and Kanamycin gene provide selection of antibiotics puromycin and kanamycin, respectively. There is an integrated turboGFP element driven by a cMV promoter to readily verify transfection efficiency.
  • U6_F Primer can be used to sequence the target sequence after cloning.
• Download Vector Sequence:
• Components: 1 vial of 5 ug lyophilized pGFP-V-RS shRNA cloning vector
• Quality Control: This vector was sequence verified and functionally validated.
• Storage: Store the vector DNA at -20°C.
• Stability: Vector DNA is stable for at least one year from date of receipt when stored at -20°C.