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pCMV6-AN-Myc-DDK-AC-His Mammalian Expression Vector

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SKU
PS100111
pCMV6-AN-Myc-DDK-AC-His, mammalian expression vector with N-terminal Myc-DDK tags and C-terminal His tag, 10 ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag His
N-Tag Myc-DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins double fusion tags: N-terminal Myc-DDK and C-terminal His.
  • The dual-tag would allow more options for purification and detection.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Hypoxia-induced inhibin promotes tumor growth and vascular permeability in ovarian cancers
Horst, Pradhan, Chaudhary et al
Commun Biol (2022) 5 (1), 536 DOI: 10.1038/s42003-022-03495-6
Cited by 18
AI Summary: OriGene Technologies contributed to this study by providing the human ALK4 plasmid with C-terminal myc-DDK tags (pCMV6-ALK4), which was used to investigate the molecular mechanisms underlying inhibin's role in ovarian cancer progression.
Snippet: Human ALK4 with C-terminal myc-DDK tags in pCMV6 was obtained from OriGene Technologies (Rockville, MD), and subcloned into pcDNA3.1 by PCR followed by restriction digest and re-ligation. A stop codon was i
Caspase cleavage and nuclear retention of the energy sensor AMPK-α1 during apoptosis
Cheratta, Thayyullathil, Hawley et al
Cell Rep (2022) 39 (5), 110761 DOI: 10.1016/j.celrep.2022.110761
Cited by 29
AI Summary: In this study, the OriGene Anti-DDK antibody (Cat#TA50011) was used to detect DDK-tagged proteins, enabling the analysis of AMPK-α1 cleavage and localization during apoptosis.
Snippet: 730 Anti-caspase-3 Cell Signaling Cat#9662S;RRID:AB_331439 Anti-AMPK-α1/2 Cell Signaling Cat#2532L;RRID:AB_330331 Anti-PARP Cell Signaling Cat#9542L;RRID:AB_2160739 Anti-DDK OriGene Cat#TA50011;RRID:AB_2622345 Anti-Fas EMD Millipore Cat#05-201;RRID:AB_309653 Anti-H2AX Cell Signaling Cat#7631;RRID:AB_10860771 Anti-phospho-H2AX (Ser139) Cell Signaling
Showing 1-2 of 2 papers.
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