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pCMV6-AN-GFP-C-His Mammalian Expression Vector

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SKU
PS100061
pCMV6-AN-GFP-C-His, mammalian vector with N-GFP and C-His double tags, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag His
N-Tag tGFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins double fusion tags: N-terminal tGFP and C-terminal His.
  • The dual-tag would allow both visualization of the protein localization and purification with nickel affinity columns.
  • tGFP_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Long Non-Coding KCNQ1OT1 Promotes Oxygen-Glucose-Deprivation/Reoxygenation-Induced Neurons Injury Through Regulating MIR-153-3p/FOXO3 Axis
Wang, Tang, Huang et al
J Stroke Cerebrovasc Dis (2020) 29 (10), 105126 DOI: 10.1016/j.jstrokecerebrovasdis.2020.105126
Cited by 28
AI Summary: In this study, OriGene products—specifically the KCNQ1OT1 siRNA and FOXO3 expression plasmid—were used to manipulate gene expression in neurons, enabling the authors to investigate the roles of KCNQ1OT1 and FOXO3 in oxygen-glucose deprivation/reoxygenation-induced neuronal injury.
Activation of the pro-migratory bone morphogenetic protein receptor 1B gene in human MDA-MB-468 triple-negative breast cancer cells that over-express CYP2J2
Allison, Chen, Petrovic et al
Int. J. Biochem. Cell Biol. (2016) 80, 173-178 DOI: 10.1016/j.biocel.2016.10.004
AI Summary: OriGene's CYP2J2 expression plasmid was used to stably transfect MDA-MB-468 breast cancer cells, enabling the study of how CYP2J2 overexpression influences gene expression and cell migration in this research.
Snippet: The MDA-MB-468 breast cancer cell line was obtained from ATCC (Manassas,VA) and was stably transfected with CYP2J2 and GFP expression plasmids (OriGene, Rockville,MD and pEGFP-N1, Invitrogen, Mulgrave, VIC, Australia, respectively) under the control of ... Cite SaveSaving
Showing 1-2 of 2 papers.
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