ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins with 3 tandem DDK-tags on the C-terminal ends, resulting in 100X higher detection sensitivity than 1XDDK tag
The 3DDK tag will increase the affinity of the fusion protein to anti-DDK tags and facilitate detection. This is especially useful for proteins of low expression level.
If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal 3DDK-tagging vector, PS100058.
V2_F Primer can be used to sequence the target sequence after cloning.
XL39_R Primer can be used to sequence the target sequence after cloning.
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