OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

pCMV6-AC-3DDK Mammalian Expression Vector

Be the first to review this product
SKU
PS100057
pCMV6-AC-3DDK, mammalian vector with C-terminal 3XDDK tag, 10ug
  $670.00
In Stock*

Frequently Bought Together (3)

GE100111 Forward primer to sequence targets cloned in expression vectors.
1 nmol
$71.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00
Specifications
Specifications
Product Data
C-Tag 3DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins with 3 tandem DDK-tags on the C-terminal ends, resulting in 100X higher detection sensitivity than 1XDDK tag
  • The 3DDK tag will increase the affinity of the fusion protein to anti-DDK tags and facilitate detection. This is especially useful for proteins of low expression level.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal 3DDK-tagging vector, PS100058.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping Ambient
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

RSPO2 Coordinates with GDF9:BMP15 Heterodimers to Promote Granulosa Cell and Oocyte Development in Mice
Wang, Li, You et al
Adv Sci (Weinh) (2025)
Cited by 9
AI Summary: OriGene’s pCMV6-AC-3DDK vector (Cat# PS100057) was used to clone coding regions for transfection experiments in mouse granulosa cells, enabling the functional studies of RSPO2 and its coordination with GDF9:BMP15 in oocyte and granulosa cell development.
Snippet: 6 coding regions were cloned into the pCMV6-AC-3DDK vector (Cat# PS100057, Origene, Rockville, Maryland, USA). Mouse granulosa cells at 50–60% confluence were transfected with 1.0 µg of each plasmid using Lipo6000 transfection reagent (Cat # C0526, Beyotime, China). …
LSM14B is an Oocyte-Specific RNA-Binding Protein Indispensable for Maternal mRNA Metabolism and Oocyte Development in Mice
Li, Zhao, Yang et al
Adv Sci (Weinh) (2023) 10 (18), e2300043 DOI: 10.1002/advs.202300043
Cited by 33
AI Summary: OriGene's pCMV6-AC-3DDK vector and pCMV6-AN-mKate plasmid were used to generate reporter constructs for functional assays investigating the role of LSM14B in maternal mRNA regulation during mouse oocyte development.
Snippet: To prepare the construct for this assay, the sequence of fluorescent protein mKate was first amplified by PCR using the pCMV6‐AN‐mKate plasmid DNA as template, and cloned into pCMV6‐AC‐3DDK (Origene, USA) vector. The resulted pCMV6‐AC‐mKate‐3DDK construct was then used as the destination vector for cloning the Mastl‐3′UTR. Mastl‐3′UTR was amplified by PCR using the mouse ooc;- Mammalian oogenesis ...
SARS-CoV-2 spike protein enhances MAP4K3/GLK-induced ACE2 stability in COVID-19
Chuang, Hsueh, Hsu et al
EMBO Mol Med (2022) 14 (9), e15904 DOI: 10.15252/emmm.202215904
Cited by 24
AI Summary: OriGene’s pCMV6-AC-3DDK vector was used to clone and express human ACE2 cDNA and its mutants, enabling the study of ACE2 stability and function in the context of SARS-CoV-2 infection in this research.
Snippet: tagged or 3xMyc‐tagged human ACE2 cDNA (NCBI accession number: NM_021804.3) were generated by cloning the cDNA from HCC827 cells by RT–PCR and then subcloning into the vector pCMV6‐AC‐3DDK (Origene) or pCMV‐3Tag‐9 (Agilent Technologies). The plasmids encoding ACE2 (S776A), ACE2 (S783A), ACE2 (S776/783A), ACE2 (S776E), ACE2 (S783E), ACE2 (S776/783E), and ACE2 (Y781F) were generated by
Compounds targeting OSBPL7 increase ABCA1-dependent cholesterol efflux preserving kidney function in two models of kidney disease
Wright, Varona Santos, Kemmer et al
Nat Commun (2021) 12 (1), 4662 DOI: 10.1038/s41467-021-24890-3
Cited by 62
AI Summary: OriGene products, specifically the OSBPL7 Myc-DDK-tagged expression plasmid and the anti-DDK (FLAG) antibody, were used in this study to overexpress and detect OSBPL7 protein, enabling the identification of OSBPL7 as the molecular target of novel compounds that enhance cholesterol efflux and protect kidney function.
FigureFigureFigure
Echinoderm Microtubule Associated Protein Like 1 Is Indispensable for Oocyte Spindle Assembly and Meiotic Progression in Mice
Yin, Zhang, Wang et al
Front Cell Dev Biol (2021) 9, 687522 DOI: 10.3389/fcell.2021.687522
Cited by 17
AI Summary: In this study, OriGene’s EML1-specific morpholinos (Gene-specific morpholino oligos) were used to knock down EML1 expression in mouse oocytes, enabling the authors to investigate EML1’s essential role in oocyte spindle assembly and meiotic progression.
FigureFigureFigure
DUSP11 Attenuates Lipopolysaccharide-Induced Macrophage Activation by Targeting TAK1
Yang, Chuang, Tsai et al
J. Immunol. (2020)
Cited by 18
AI Summary: OriGene products contributed to this study by providing the pCMV6-AC-3DDK vector used to construct the Flag-DUSP11 plasmid and the anti-Flag (DDK) antibody for protein detection.
Snippet: Plasmids, Abs, and reagents Flag-DUSP11 plasmid was constructed by subcloning human DUSP11 cDNA into the vector pCMV6-AC-3DDK (OriGene Technologies) Anti-Flag (DDK) Ab was purchased from Origene Technologies. Anti-vinculin Ab was purchased from
Showing 1-6 of 10 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products