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pCMV6-AN-FC Mammalian Expression Vector

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SKU
PS100055
pCMV6-AN-FC, mammalian vector with N-terminal FC tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag FC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal murine Fc tag.
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal Fc-tagging vector, PS100053.
  • FC_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization
Hauler, Mallery, McEwan et al
PNAS (2012) 109 (48), 19733-8 DOI: 10.1073/pnas.1210659109
Cited by 105
AI Summary: In this study, the Myc-DDK–tagged mouse VCP plasmid (MR210760, OriGene) was used to overexpress VCP in HeLa cells, enabling investigation of VCP's essential role in TRIM21-mediated virus neutralization.
Snippet: 1 D and E) against human VCP or with Silencer Negative Control siRNA #1 (Ambion). For overexpression of mouse VCP, HeLa cells were transiently transfected with a plasmid encoding the Myc-DDK–tagged protein (MR210760, OriGene) or with empty vector.
Showing 1-1 of 1 papers.
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