pCMV6-AN-FC Mammalian Expression Vector

SKU PS100055

pCMV6-AN-FC, mammalian vector with N-terminal FC tag, 10ug

Product Data

• N-Tag: FC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Expression Promoter: CMV
• Features:
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal murine Fc tag.
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal Fc-tagging vector, PS100053.
  • FC_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
• Shipping: Ambient