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pCMV6-AC-FC-S Mammalian Expression Vector

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SKU
PS100054
pCMV6-AC-FC-S, mammalian vector with C-terminal FC and secretion signal, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag FC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a secreted tagged protein with a C-terminal murine Fc tag. Due to the presence of an N-terminal secretion signal sequence, the fusion protein will be secreted and this facilitates the downstream purification
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Fc-tagging vector, PS100056
  • Validation Data
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • FC_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

VSIG2 as a novel immunosuppressive ligand interacts with Nectin-2 to regulate T cell responses
Wang, Hu, Chen et al
J Neuroinflammation (2025)
Cited by 1
AI Summary: OriGene’s pCMV6-AC-FC-S expression vector (Cat. No. PS100054) was used to clone and express the extracellular domain of hVSIG2, enabling the study of its immunosuppressive interactions with Nectin-2 in T cell regulation.
Snippet: The extracellular structural domain of hVSIG2 (aa24-243) was 790 cloned and fused into the pCMV6-AC-FC-S expression vector 791 (ORIGENE, Cat. No. PS100054, Rockville, MD) containing a constant 792 region of murine IgG2a. The vector was then transfected into HEK-293 793 F cells (Thermo Fisher Scientific, Cat. No. R79007) to establish a stable
Improving African Swine Fever Surveillance Using Fluorescent Rapid Tests
Aira, Monedero, Hernández-Antón et al
Pathogens (2023) 12 (6) DOI: 10.3390/pathogens12060811
Cited by 8
AI Summary: OriGene's pCMV6-AC-Fc-S expression vector was used to clone and express the recombinant antibody (scFv-Fc) against VP72, which was essential for developing the improved fluorescent rapid tests for African swine fever surveillance described in the study.
Snippet: n variable fragment (scFv) construct connecting 3′VH to 5′VL through the linker. This scFv was cloned by HindIII/Xho I digestion and subsequently ligated into the expression vector pCMV6-AC-Fc-S (Origene, Rockville, MD, USA) that adds a mouse Fc tag at the 3′-end of the cloned sequence. Hence, the final format of the rMAb was a scFv-Fc. The cloned sequence was corroborated using automatic s
FigureFigureFigure
Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
Fresco-Taboada, García-Durán, Aira et al
Diagn Microbiol Infect Dis (2022) 102 (4), 115650 DOI: 10.1016/j.diagmicrobio.2022.115650
Cited by 5
AI Summary: In this study, OriGene’s SARS-CoV-2 recombinant Receptor Binding Domain (RBD) protein was used as a key antigen in the development of the ELISA assay to evaluate the presence of virus-specific antibodies in serum samples following vaccination.
FigureFigureFigure
Recombinant CD300c-Fc fusion protein attenuates collagen-induced arthritis in mice
Liu, Zhao, Su et al
Rheumatology (Oxford) (2021)
Cited by 9
AI Summary: OriGene’s pCMV6-AC-FC-S expression vector was used to clone the extracellular domains of human CD300c, enabling the production of the recombinant CD300c-IgG2a Fc fusion protein that was central to the study’s investigation of arthritis treatment in mice.
Snippet: Connecticut. Production of recombinant human CD300c-Ig fusion protein The extracellular domains of human CD300c was cloned into a pCMV6-AC-FC-S expression vector containing the constant region of mouse IgG2a (ORIGENE). The vector was
Identification of TAPBPL as a novel negative regulator of T-cell function
Lin, Cui, Su et al
EMBO Mol Med (2021) 13 (5), e13404 DOI: 10.15252/emmm.202013404
Cited by 21
AI Summary: OriGene's pCMV6-AC-FC-S expression vector was used to clone and express the extracellular domain of human and mouse TAPBPL, enabling the production of fusion proteins essential for the study's functional analyses.
Snippet: o). Cloning and purification of TAPBPL The extracellular domain of hTAPBPL or mTAPBPL was cloned and fused into a pCMV6‐AC‐FC‐S expression vector containing the constant region of mouse IgG2a (ORIGENE, Rockville, MD). The vector was transfected into HEK‐293 cells. The fusion proteins were purified from the supernatant using Protein G Sepharose 4 Fast Flow according to the manufacturer’s;The extra...
FigureFigureFigure
IP
Unveiling a novel function of CD9 in surface compartmentalization of oocytes
Inoue, Saito, Wada
Development (2020)
Cited by 33
AI Summary: OriGene’s pCMV6-AC-FC-S expression vector was used to generate fusion proteins for analyzing molecular profiles of CD9 and JUNO in oocytes, contributing to the study’s investigation of CD9’s role in surface compartmentalization.
Snippet: region (residues 1-21) was expressed fusing with the CH2 and CH3 domains of the mouse IgG2a heavy chain and the hinge region from the expression vector pCMV6-AC-FC- S (OriGene). This construct uses a human interleukin-2 (IL-2) leader sequence at the N terminus
Showing 1-6 of 16 papers.
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