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pCMV6-AN-FC-S Mammalian Expression Vector

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SKU
PS100056
pCMV6-AN-FC-S, mammalian vector with N-terminal FC and secretion signal, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag FC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a secreted tagged protein with an N-terminal murine Fc tag. Due to the presence of an N-terminal secretion signal sequence, the fusion protein will be secreted and this facilitates the downstream purification.
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal Fc-tagging vector, PS100054.
  • Validation Data
  • FC_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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