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pCMV6-AN-mGFP Mammalian Expression Vector

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SKU
PS100048
pCMV6-AN-mGFP, mammalian vector with N-terminal mGFP, 10ug
  $670.00
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Specifications
Specifications
Product Data
N-Tag mGFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mGFP (monomeric) tag.
  • mGFP (scientific name mTagGFP) is the improved variant of tagGFP, the mutant of the Aequorea macrodactyla GFP-like protein. mGFP possesses bright green fluorescence with excitation/emission maxima at 483 and 506 nm, respectively.
    Compared to EGFP, mGFP provides about the same brightness of fluorescence but is significantly more pH stable. mGFP is specially optimized for expression at 37°C.
    Because of monomeric nature, mGFP is mainly intended for protein localization studies and expression in long-term cell cultures. In FRET applications, mGFP can be used as a donor for red fluorescent protein mRFP or as an acceptor for blue fluorescent protein mBFP.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal mGFP-tagging vector, PS100040.
  • mGFP_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Detection by super-resolution microscopy of viral proteins inside bloodborne extracellular vesicles
Singh, Santos, Herndon et al
Extracell Vesicles Circ Nucleic Acids (2023) 4 (4), 557-67 DOI: 10.20517/evcna.2023.46
Cited by 19
Snippet: Using Lipofectamine 3000 (#L3000008, Thermo Fisher Scientific), FEMX-I cells were transfected with the pCMV6-AN-mGFP mammalian expression vector (#PS100048, OriGene) to express green fluorescent protein (GFP). Transfected cells were selected by introducing 400 µg/mL of Geneticin (G418 Sulfate, #10131027, Thermo Fisher Scientific) into the culture medium. Selection antibiotics were removed from the
The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
Dunlevy, Medvedeva, Wilson et al
J Cell Sci (2020) 133 (10) DOI: 10.1242/jcs.240416
Cited by 28
AI Summary: In this study, OriGene products, specifically the PRR14 Myc-DDK tagged cDNA (OriGene RC224601) and PPP2R1A Myc-DDK tagged cDNA (OriGene RC200176), were used to express and analyze the roles of PRR14 and PPP2R1A in investigating the mechanisms of nuclear lamina targeting and regulation.
FigureFigureFigure
MAP4K3/GLK promotes lung cancer metastasis by phosphorylating and activating IQGAP1
Chuang, Chang, Teng et al
Cancer Res. (2019)
AI Summary: OriGene products, specifically the pCMV6-AN-3DDK, pCMV6-AC-HA, pCMV6-AC-mCFP, and pCMV6-AN-mGFP expression vectors, were used to generate tagged GLK constructs for studying the interaction and functional effects of GLK and IQGAP1 in lung cancer metastasis.
Snippet: the Flag-tagged clone into the vector pCMV6-AN-3DDK or pCMV6-AC-HA (OriGene,) insert from the HA-tagged GLK plasmid into the vector pCMV6-AC-mCFP and pCMV6-AN-mGFP (OriGene), respectively. The plasmids expressing YFP-fused
Domain analysis of PNKP/XRCC1 interactions: influence of genetic variants of XRCC1
Mani, Mermershtain, Abdou et al
J. Biol. Chem. (2018)
AI Summary: OriGene’s pEX-N-His bacterial expression plasmid was used to clone and express full-length XRCC1 and its 166-436 residue fragment, enabling the study of XRCC1-PNKP interactions and the functional impact of XRCC1 genetic variants in this research.
Snippet: The selected colonies were checked by DNA sequence verification. The cDNA encoding full- length XRCC1 and residues 166-436 was amplified by PCR and inserted into the bacterial expression plasmid pEX-N-His (OriGene, Rockville, MD) using AscI-RsrII restriction
Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1
Meyer, Dunn, Vidler et al
FASEB J. (2018)
AI Summary: OriGene’s MCPH1-L expression plasmid (PS100048) was used to generate an N-terminal GFP-tagged MCPH1-L construct, enabling the study of MCPH1 isoform regulation during mitosis in this research.
Snippet: Texas, USA). This plas- mid was used as a template to subclone MCPH1-L into an ex- pression plasmid with an N-terminal green fluorescent protein (GFP) tag (PS100048;OriGene Technologies, Rockville, MD, USA). Plasmids
The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network
Jiang, Li, Poleshko et al
EBioMedicine (2017) 22, 28-43 DOI: 10.1016/j.ebiom.2017.06.024
Cited by 37
AI Summary: In this study, OriGene products—including the CCDC170 Myc-DDK-tagged cDNA (RC210885) and CCDC170-specific shRNA (TL312285)—were used to manipulate CCDC170 gene expression, enabling the authors to investigate its role in organizing the Golgi-microtubule network in breast cancer cells.
FigureFigureFigure
Showing 1-6 of 20 papers.
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