pCMV6-AC-mGFP Mammalian Expression Vector

Snippet: pCMV6-AC-tGFP plasmid vector encoding EIF2B5 and EIF2S1 was purchased from 697 OriGene (Rockville, Maryland, USA). The coding open reading frame of EIF2B5 from 698 the pCMV6-AC-tGFP vector was cloned into an empty pCMV6-AC-mGFP and empty 699 pCMV6-AC-mRFP vectors. The constructs were verified by sequencing

Snippet: ional NES responsible for relocalizing NPM to the cytoplasm. Expression of mutant PNKP proteins revealed several consequences that together raise the possibility that PNKP mutations could lead to tumorigenesis as well as MCSZ. As seen with other PNKP mutations, the level of the mutant proteins, particularly the T323M PNKP, was significantly depressed. Taking this into account together with its se

Snippet: ;The vector expressing SOX9-mGFP was constructed by cloning mGFP (PS100040, Origene) with the C-terminal of wild type SOX9 (RC208944, Origene) using SgfI and MluI restriction sites. … Non-dimerizing and non-DNA binding mutated SOX9-mGFP, A76E and W143R respectively, were generated from SOX9-mGFP by site-directed mutagenesis (210518, Agilent). …

Snippet: 7 °C in 5% CO2 in a humidified incubator overnight before further manipulations. LACTATE ASSAY IN HEPG2 AND HPH CELLS HepG2 cells were cotransfected with plasmids expressing human GCK (SC127236, Origene) and GKRP (SC119244, Origene) or GCK and the control vector pCMV6-XL4 (Origene) in 1:0, 1:1 and 1:3 plasmid molar ratios (_GCK_ to _GCKR_;_GCK_ to pCMV6-XL4). All transfections were carried o

Snippet: Plasmids, Mutagenesis and Lentiviral Production  The full-length human TRPV4 (NM_021625) cDNA clone was obtained from Origene (Rockville, MD) and shuttled into a mammalian expression vector resulting in a TRPV4 fusion protein with its COOH-terminus tagged with turbo GFP