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pCMV6-AC-mCFP Mammalian Expression Vector

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SKU
PS100044
pCMV6-AC-mCFP, mammalian vector with C-terminal mCFP, 10ug
  $670.00
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Specifications
Specifications
Product Data
C-Tag mCFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mCFP (monomeric) tag.
  • mCFP is a cyan monomeric protein. It possesses bright fluorescence with excitation/emission maxima at 458 and 480 nm, respectively. mCFP is significantly brighter than commonly used ECFP. mCFP is mainly intended for protein labeling in protein localization and interaction studies. It can also be used for cell and organelle labeling and for tracking the promoter activity.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mCFP-tagging vector, PS100052.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • mCFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

MAP4K3/GLK promotes lung cancer metastasis by phosphorylating and activating IQGAP1
Chuang, Chang, Teng et al
Cancer Res. (2019)
AI Summary: OriGene products, specifically the pCMV6-AN-3DDK, pCMV6-AC-HA, pCMV6-AC-mCFP, and pCMV6-AN-mGFP expression vectors, were used to generate tagged GLK constructs for studying the interaction and functional effects of GLK and IQGAP1 in lung cancer metastasis.
Snippet: the Flag-tagged clone into the vector pCMV6-AN-3DDK or pCMV6-AC-HA (OriGene,) insert from the HA-tagged GLK plasmid into the vector pCMV6-AC-mCFP and pCMV6-AN-mGFP (OriGene), respectively. The plasmids expressing YFP-fused
GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease
Chuang, Tsai, Hsueh et al
Sci Adv (2018) 4 (9), eaat5401 DOI: 10.1126/sciadv.aat5401
AI Summary: OriGene products, specifically the pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, and pCMV6-AC-Flag vectors, were used to construct plasmids for expressing tagged proteins (such as PKCθ and AhR) that enabled the study of protein interactions and signaling mechanisms underlying IL-17A induction in autoimmune disease.
Snippet: PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …
FigureFigureFigure
PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-κB signaling in lung cancer
Ho, Liang, Liang
Oncotarget (2016) 7 (32), 52255-52269 DOI: 10.18632/oncotarget.10427
Snippet: The materials were purchased and obtained as follows: Recombinant human EGF, HGF and IGF-1 were from R&D Systems (Abingdon, UK);pCMV6-PP4R1 (NM_005134), pCMV6-PP4R2 (NM_174907), pCMV6-PP4C (NM_002720), pCMV6-PATZ1 variant 3 (NM_032052), and pCMV6-PATZ1 variant 4 (NM_032051) plasmids were from ORIGENE (Rockville, MD);
FigureFigureFigure
Showing 1-3 of 3 papers.
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