pCMV6-AC-mBFP Mammalian Expression Vector

SKU PS100043

pCMV6-AC-mBFP, mammalian vector with C-terminal mBFP, 10ug

Product Data

• C-Tag: mBFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Expression Promoter: CMV
• Features:
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mBFP (monomeric) tag.
  • mBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein. mBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.; Compared to EBFP2, mBFP is more than 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of mBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.; Good overlap between the emission spectrum of mBFP and the absorbance spectra of mGFP allows using these two proteins as a FRET pair.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mBFP-tagging vector, PS100051.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • mBFP_R Primer can be used to sequence the target sequence after cloning.
• Shipping: Ambient