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pCMV6-AC-mKate Mammalian Expression Vector

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SKU
PS100039
pCMV6-AC-mKate, mammalian vector with C-terminal mKate tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
C-Tag mKate
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mKate2 tag.
  • mKate2 possesses fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at physiological pH 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date.
    mKate2 is characterized by complete and fast chromophore maturation at 37°C with maturation half-time <20 min (versus 40 min for mCherry). It is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including TagFP635, mRaspberry and mPlum. The high brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues.
    mKate2 is mainly intended for protein labeling. Its far-red fluorescence allows easy and reliable separation from standard green fluorescent labels in dual-color high-throughput assays.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mKate2 -tagging vector, PS100047
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • mKate_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
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Phosphodiesterase 5 inhibition improves contractile function and restores transverse tubule loss and catecholamine responsiveness in heart failure
Lawless, Caldwell, Radcliffe et al
Sci Rep (2019) 9 (1), 6801 DOI: 10.1038/s41598-019-42592-1
AI Summary: OriGene products contributed to this paper by providing the human BIN1 (AmpII) variant 8 cloned into the pCMV6-AC-mKate2 entry vector and the pCMV6-mKate2 control vector, which were used for transfection experiments to study the molecular mechanisms underlying transverse tubule restoration in heart failure.
Snippet: NRVMs (1–3 days post isolation) were transiently transfected with either variant 8 of human BIN1 (AmpII) (Origene Inc, USA) cloned into pCMV6-AC-mKate2 entry vector (Origene Inc, USA) or pCMV6-mKate2 as a negative control
FigureFigureFigure
A missense mutation in SLC26A3 is associated with human male subfertility and impaired activation of CFTR
Wedenoja, Khamaysi, Shimshilashvili et al
Sci Rep (2017) 7 (1), 14208 DOI: 10.1038/s41598-017-14606-3
Cited by 27
AI Summary: OriGene's PCMV6-AC-mKate vector was used to express SLC26A6 with a C-terminal mKate tag in HEK293 cells, enabling the study of protein interactions relevant to male subfertility in this research.
Snippet: HEK293 cells were transfected with slc26a3 and SLC26A3-p.Asp688His in pcDNA3.1(+) or SLC26A6 in PCMV6-AC-mKate (C-terminal mKate tag – Origene, MD)
FigureFigureFigure
Governing effect of regulatory proteins for Cl(-)/HCO3(-) exchanger 2 activity
Jeong, Hong
Channels (Austin) (2015)
Cited by 12
AI Summary: OriGene products contributed to this study by providing the CA XII cDNA in a pCMV6-AC-Myc-DDK vector and the pCMV6-AC-mKate vector, which were used to express and analyze the role of carbonic anhydrase XII in regulating AE2 activity.
Snippet: CA XII cDNA in a pCMV6-AC-Myc-DDK vector was purchased from Origene Technologies (Rockville, MD). The cDNA encoding CA XII was excised from the original vectors and transferred to pCMV6-AC-mKate vectors (Origene Technologies). ... Cite SaveSaving
Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation
Hong, Muhammad, Zheng et al
J. Physiol. (Lond.) (2015)
AI Summary: OriGene products contributed to this study by providing human wild-type CA7 and CA12 cDNA in the pCMV6-AC-Myc DDK vector, as well as the pCMV6-AC-mKate vector, which were used for gene expression and functional analyses in secretory gland research.
Snippet: Louis, MO). Human wild-type CA7 and CA12 cDNA in pCMV6-AC-Myc DDK vector was purchased from Origene Tech. (Rockville, MD). ... 7 NBCe1-B were excised from the original vectors and transferred to pCMV6-AC-mKate (Origene Tech.) and pEGFP-C1, respectively ... Cite SaveSaving
Showing 1-4 of 4 papers.
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