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pCMV6-AC-FP635 Mammalian Expression Vector

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SKU
PS100038
pCMV6-AC-FP635, mammalian vector with C-terminal FP635 tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag TurboFP635
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal TurboFP635 tag.
  • TurboFP635 (scientific name Katushka) is a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor. Possessing excitation/emission maxima at 588/635 nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close or mPlum. TurboFP635 is characterized by fast maturation and a high pH-stability and photostability. The unique characteristics of TurboFP635 make it the protein of choice for visualization within living tissues and dual-color high-throughput assays.
  • TurboFP635 is mainly intended for applications where fast appearance of far-red fluorescence is crucial. It is specially recommended for whole body imaging, cell and organelle labeling, and for tracking the promoter activity in auto-fluorescent tissues.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal FP635-tagging vector, PS100046.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • TurboFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

GlyT1 determines the glycinergic phenotype of amacrine cells in the mouse retina
Eulenburg, Knop, Sedmak et al
Brain Struct Funct (2018)
AI Summary: In this study, the FP635 fluorescent protein from OriGene was used to create a fusion protein for validating genetic constructs during the generation of GlyT1b knock-in mice, aiding in the investigation of GlyT1's role in retinal amacrine cells.
Snippet: Generation of GlyT1b KI mice A cDNA fragment encoding for a fusion protein (LucR) of the firefly luciferase (Promega) and FP635 (Origene) was generated by conventional cloning strategies and tested by transient transfection in HEK293 cells
Showing 1-1 of 1 papers.
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