pCMV6-AC-FP635 Mammalian Expression Vector

SKU PS100038

pCMV6-AC-FP635, mammalian vector with C-terminal FP635 tag, 10ug

Product Data

• C-Tag: TurboFP635
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Expression Promoter: CMV
• Features:
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal TurboFP635 tag.
  • TurboFP635 (scientific name Katushka) is a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor. Possessing excitation/emission maxima at 588/635 nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close or mPlum. TurboFP635 is characterized by fast maturation and a high pH-stability and photostability. The unique characteristics of TurboFP635 make it the protein of choice for visualization within living tissues and dual-color high-throughput assays.
  • TurboFP635 is mainly intended for applications where fast appearance of far-red fluorescence is crucial. It is specially recommended for whole body imaging, cell and organelle labeling, and for tracking the promoter activity in auto-fluorescent tissues.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal FP635-tagging vector, PS100046.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • TurboFP_R Primer can be used to sequence the target sequence after cloning.
• Shipping: Ambient