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pCMV6-AC-IRES-GFP Mammalian Expression Vector

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SKU
PS100027
pCMV6-AC-IRES-GFP, mammalian vector with IRES-driven tGFP expression, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed as a tagged protein with a C-terminal Myc-DDK tag.
  • TurboGFP is expressed from IRES as part of the bicistronic transcript.
  • TurboGFP (excitation/ emission max = 482/ 502 nm) is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboGFP
  • Such clones are the best for detection and purification of the transgene expression using an anti-Myc and anti-DDK antibody.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • IRES_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

ASXL1 deficiency causes epigenetic dysfunction, combined immunodeficiency, and EBV-associated lymphoma
Fu, Sharma, Yousefi et al
J Exp Med (2025) 222 (10) DOI: 10.1084/jem.20240945
Cited by 2
Snippet: Full-length WT ASXL1 cDNA (NM_015338;Cat# RG219354;OriGene Technologies) was cloned into a pCMV6-AC-IRES-GFP vector (Cat# PS100027;OriGene Technologies) using the restriction enzymes AsiSI (Cat# R0630S;New England Biolabs) and MluI-HF (Cat# R3198S;New England Biolabs). T
Untangling sources of DNA methylation variability in the context of development and rare diseases
Fu
Thesis (2025)
AI Summary: OriGene’s pCMV6-AC-IRES-GFP vector (Cat# PS100027) was used to clone full-length ASXL1 cDNA, providing a platform for generating and studying ASXL1 variants in this research.
Snippet: Full-length ASXL1 cDNA was cloned into a pCMV6-AC-IRES-GFP vector (Cat# PS100027;OriGene Technologies, Rockville, MD, USA) using the following restriction enzymes AsiSI (Cat# R0630S;New England Biolabs, Ipswich, MA, USA) and MluI-HF (Cat# R3198S;New England Biolabs). The p.C111Y and p.S1237F variants reported in this study were generated in the cloned vector through site-directed mutagenesis
Activated Src kinases downstream of BCR-ABL and Flt3 induces proteasomal degradation of SHIP1 by phosphorylation of tyrosine 1021
Ehm, Bettin, Jücker
Biochim Biophys Acta Mol Cell Res (2023) 1870 (5), 119467 DOI: 10.1016/j.bbamcr.2023.119467
Cited by 9
AI Summary: OriGene contributed the pCMV6-AC-IRES-GFP vector (No. PS100027) to this study, which was used in the construction of expression plasmids for investigating the molecular mechanisms regulating SHIP1 degradation.
Snippet: Within the various subtypes of ALL, patients with a BCR-ABL-positive background as well as with a genetic change in the KMT2A gene have by far the worst survival Save;pCMV6-AC-IRES-GFP vector was received from Origene (No. PS100027). Src cDNA was received from PlasmID (clone: HsCD00021252). Construction of this construct was carried out by PCR cloning
Role of Oatp2b1 in Drug Absorption and Drug-Drug Interactions
Chen, Hu, Li et al
Drug Metab Dispos (2020) 48 (5), 419-425 DOI: 10.1124/dmd.119.090316
Cited by 41
AI Summary: In this study, OriGene's OATP2B1 cDNA clone (Cat# SC119314) was used to facilitate the expression and functional characterization of the OATP2B1 transporter, enabling the assessment of its role in drug absorption and drug-drug interactions.
Metalloprotease inhibitor profiles of human ADAM8 in vitro and in cell-based assays
Schlomann, Dorzweiler, Nuti et al
Biol Chem (2019) 400 (6), 801-810 DOI: 10.1515/hsz-2018-0396
Cited by 33
AI Summary: In this study, OriGene’s recombinant human ADAM8 protein (Cat# TP317435) and human ADAM8 cDNA clone (Cat# RC210865) were used to assess ADAM8 enzymatic activity and its inhibition profile in both in vitro and cell-based assays.
Plasma cell deficiency in human subjects with heterozygous mutations in Sec61 translocon alpha 1 subunit (SEC61A1)
Schubert, Klein, Hassdenteufel et al
J Allergy Clin Immunol (2018) 141 (4), 1427-1438 DOI: 10.1016/j.jaci.2017.06.042
Cited by 83
AI Summary: In this study, OriGene products—specifically the SEC61A1 cDNA clone and SEC61A1 shRNA—were used to manipulate SEC61A1 expression in cell lines, enabling functional analysis of the identified SEC61A1 mutations and their impact on plasma cell differentiation and survival.
FigureFigure
Showing 1-6 of 16 papers.
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