pCMV6-A-GFP Mammalian Expression Vector

Snippet: ty Kit Thermo Fisher Scientific Cat# TR22421 TurboFect Thermo Fisher Scientific Cat# R0532 Recombinant DNA lentiCRISPR v2 Addgene Cat# 52961 pCMV6-entry-GFP Origene Cat# PS100026 pCMV-ADH1B WT-Flag Origene Cat# RC205391L3 pCMV-ADH1B c.937C>T-Flag Described in Methods N/A Software and Algorithms Multi-Experiment Viewer (MeV)

Snippet: sequence was submitted to the BepiPred-2.0: Sequential B-Cell Epitope Predictor Immune Epitope Database tool. The E1/T2A/E2 sequence was synthesized and inserted in a mammalian expression construct (OriGene PS100026;OriGene, Rockville, MD, USA) under the control of a cytomegalovirus promotor (synthesized via Blue Heron Biotech LLC, Bothell, WA, USA) (envelope epitopes construct abbreviated as EC

Snippet: Ltd. pCMV6-A-GFP, pGFP-V-RS and pMirTarget-3'UTR pMirTarget-3'UTR were purchased from Origene (Rockville, MD,USA). pCMV6-A-GFP-MEG3, pGFP-V-RS-C- MEG3, pGFP-V-RS-TERT and pMartarget-3'UTR-C-TERT promoter were cloned by ourselves

Snippet: Plasmid-containing human QPRT (pCMV6-QPRT-Myc-DDK-tagged clone;OriGene) or control GFP (2 μg) (pCMV6-A-GFP-tagged cloning vector;OriGene) were incubated with a mixture of nucleofection solution and P4 primary cell supplement (82 μl:18 μl nucleofection solution:supplement) and placed in nucleofection cuvettes

Snippet: with 10% heat-inactivated fetal bovine serum (Gibco) in a humidified atmosphere of 5% CO 2 incubator at 37 °C. Plasmid pGFP-V-RS, pCMV6-A-GFP, pCMV6-XL5-PTEN, pGFP-V-RS-PTEN, pGFP-V-RS-Sirt1, pMiR-Target were purchased from Origene (Rockville Cached