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pCMV6-A-BSD Mammalian Expression Vector

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SKU
PS100022
pCMV6-A-BSD, mammalian vector with blasticidin as mammalian selection marker, 10ug
  $470.00
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Specifications
Specifications
Product Data
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Blasticidin
Expression Promoter CMV
Features
  • This vector is essentially the same as the pCMV6-Entry vector, except for the alternative selection marker (Blasticidin instead of Neomycin).
  • The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Tissue-specific mitochondrial HIGD1C promotes oxygen sensitivity in carotid body chemoreceptors
Timón-Gómez, Scharr, Wong et al
Elife (2022) 11 DOI: 10.7554/eLife.78915
AI Summary: OriGene products HP234519 (TH-F and TH-R primers) were used in this study to analyze gene expression related to oxygen sensing in carotid body chemoreceptors.
Snippet: This paper COX4I2-R: CTTCTCCTTCTCCTTCAGGG This paper NDUFA4L2-F: GATGATCGGCTTAATCTGCC This paper NDUFA4L2-R: GTATTGGTCATTGGGGCTCA This paper TH-F: GCTGGACAAGTGTCATCACCTG OriGene HP234519 TH-R: CCTGTACTGGAAGGCGATCTCA OriGene HP234519 OR51E2-F2: TCATCCCATTGTGCGTGTTG This paper OR51E2-R2: CACCCGTGTTCTGATCTGTTTG This paper   BaseScop
FigureFigureFigure
Coordination of metal center biogenesis in human cytochrome c oxidase
Nývltová, Dietz, Seravalli et al
Nat Commun (2022) 13 (1), 3615 DOI: 10.1038/s41467-022-31413-1
Cited by 120
AI Summary: OriGene's CRISPR-CAS9 guide RNAs (KN210770 and KN203238) were used to generate stable COX19 and COX11 knockout HEK293T cell lines, enabling the study of metal center biogenesis in human cytochrome c oxidase.
Snippet: … To create stable human COX19-KO and COX11-KO lines in HEK293T cells, we used CRISPR-CAS9 guide RNAs obtained from OriGene (KN210770 and KN203238, respectively) designed to bind the exon 1 region of the corresponding gene (Supplementary Fig. 1). 1.5 × 106 HEK293T cells were transfected with 4 µg of the r;- To create stable human COX19-KO and COX11-KO lines in HEK293T cells, we used CRISPR-CAS9 guide RNAs obtained from OriGene (KN210770 and KN203238,
Coupled transmembrane mechanisms control MCU-mediated mitochondrial Ca2+ uptake
Vais, Payne, Paudel et al
Proc Natl Acad Sci U S A (2020) 117 (35), 21731-21739 DOI: 10.1073/pnas.2005976117
Cited by 60
AI Summary: OriGene products, specifically the MICU1 (Cat# RC209545) and MICU2 (Cat# RC217768) cDNA clones, were used in this study to express and analyze the roles of MICU1/2 in regulating MCU-mediated mitochondrial Ca2+ uptake.
PFKFB2 regulates glycolysis and proliferation in pancreatic cancer cells
Ozcan, Sarioglu, Altunok et al
Mol. Cell. Biochem. (2020) 470 (1-2), 115-129 DOI: 10.1007/s11010-020-03751-5
Cited by 49
AI Summary: OriGene’s FLAG-tagged cDNA plasmids for PFKFB2 mRNA splice variant 1 (Cat.# RC211329) and variant 2 (Cat.# RC219676) were used to express and study the functional roles of these PFKFB2 variants in regulating glycolysis and proliferation in pancreatic cancer cells.
Snippet: FLAG-tagged cDNA plasmids of PFKFB2 mRNA splice variant 1 (Origene;Cat.# RC211329) and variant 2 (Ori- gene;Cat.# RC219676) were linearized with BamH1 and Pme1 enzymes and the resulting DNA fragments were then ligated into the BamH1/Pme1-cut pCMV6;- FLAG-tagged cDNA plasmids of PFKFB2 mRNA splice variant 1 (Origene
Discovery and Characterization of VU0529331, a Synthetic Small-Molecule Activator of Homomeric G Protein-gated, Inwardly-rectifying, Potassium (GIRK) Channels
Kozek, Du, Sharma et al
ACS Chem Neurosci (2018) 10 (1), 358-370 DOI: 10.1021/acschemneuro.8b00287
AI Summary: OriGene’s pCMV6-A-BSD (KCNJ3) and pCMV6-A-puro (KCNJ6) expression vectors were used to generate HEK293 cells co-expressing human GIRK1 and GIRK2 subunits, enabling functional studies of GIRK channel activation in this research.
Snippet: HEK293 cells expressing both human GIRK1 and human GIRK2 subunits were generated by transfecting a pCMV6-A-BSD vector (Origene, Rockville, MD) containing KCNJ3 and a pCMV6-A-puro vector (Origene, Rockville, MD) with KCNJ6 into low-passage HEK293 cells (CRL-1573, ATCC, Manassas, VA)
Heparin at physiological concentration can enhance PEG-free in vitro infection with human hepatitis B virus
Choijilsuren, Jhou, Chou et al
Sci Rep (2017) 7 (1), 14461 DOI: 10.1038/s41598-017-14573-9
Cited by 28
AI Summary: OriGene's CMV6-NTCP-flag tag expression vector was used to generate NTCP-reconstituted HepG2 cells, enabling the study of HBV infection mechanisms in a PEG-free in vitro system.
Snippet: CMV6-NTCP-flag tag expression vector was purchased from Origene
FigureFigureFigure
Showing 1-6 of 7 papers.
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