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pCMV6-AC-Myc-DDK Mammalian Expression Vector

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SKU
PS100007
pCMV6-AC-Myc-DDK, mammalian vector with C-terminal Myc-DDK tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed as a tagged protein with the C-terminal Myc-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene expression using anti-Myc or anti-DDK antibodies
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Myc-DDK-tagging vector, PS100016.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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From Rare to Common: Genetic Insights into TLR7 Variants in a Multicentric Spanish Study on COVID-19 Severity
Antolí, Vargas-Parra, Sierra-Fortuny et al
J Clin Immunol (2025) 45 (1), 100 DOI: 10.1007/s10875-025-01892-0
Cited by 6
AI Summary: OriGene products contributed to this study by providing the TLR7 expression plasmid (RC207515) and the pCMV6-AC-Myc-DDK mammalian expression vector (PS100007), which were used to generate TLR7 constructs for functional assays assessing the impact of TLR7 variants on COVID-19 severity.
Snippet: ted with 10% (v/v) fetal bovine serum (A5256701, Thermo Fisher Scientific) and maintained at 37°C with 5% CO2. Plasmids A TLR7 vector template (pCMV6-TLR7) was generated by inserting TLR7 (RC207515, OriGene, Rockville, MD, USA) into a pCMV6-AC-Myc-DDK Mammalian Expression Vector (PS100007;OriGene). The In-Fusion Snap Assembly Value Bundle (638,946, Takara Bio USA, San Jose, CA, USA) was used t
FigureFigure
Clinical and electrophysiological features of SCN8A variants causing episodic or chronic ataxia
Lyu, Boßelmann, Johannesen et al
eBioMedicine (2023) 98, 104855 DOI: 10.1016/j.ebiom.2023.104855
Cited by 27
AI Summary: OriGene’s human NaV1.6 channel α-subunit (PS100007) was used as the template for mutagenesis to generate TTX-insensitive SCN8A variants, enabling functional studies of disease-associated mutations in this research.
Snippet: … Mutagenesis Mutagenesis was performed as previously described, the human NaV1.6 channel α-subunit (Origene, PS100007) was modified to be tetrodotoxin (TTX, a neurotoxin blocking NaV channels in nerve cells) insensitive by introducing a point mutation (c.1112 A > G, p.Y371C).17 Ataxia-related SCN;Mutagenesis was performed as previously described, the human NaV1.6 channel α-subunit (Origene, PS100007) was modified to be tetrodotoxin (TTX, a neurotoxin blocking NaV channels in nerve cells) insensitive by introducing a point mutation (c.1112 A G, p.Y371C)
Ultrarare Loss-of-Function Mutations in the Genes Encoding the Ionotropic Glutamate Receptors of Kainate Subtypes Associated with Schizophrenia Disrupt the Interaction with PSD95
Hu, Wu, Hsu et al
J Pers Med (2022) 12 (5) DOI: 10.3390/jpm12050783
Cited by 13
AI Summary: OriGene products, including pCMV6-Myc-DDK-tagged GRIK1 (RC222898), GRIK2 (RC222369), DLG4 (RC215178) plasmids, and the pCMV6-Myc-DDK and pCMV6-AC-GFP vectors, were used to express and study the functional effects of mutant and wild-type kainate receptor genes in cell-based assays for this schizophrenia research.
Snippet: … Gene Constructs and Transient Transfection of Cell Lines pCMV6-Myc-DDK-tagged GRIK1 (RC222898), GRIK2 (RC222369), and DLG4 genes (RC215178) were purchased from Origene Technologies, Inc., Rockville, MD, USA. The cDNA encoding GRIK3, GRIK4, and GRIK5 were synthesized by MDBio, Inc. …
Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice
Segelcke, Fischer, Hütte et al
Cell Rep (2021) 37 (12), 110152 DOI: 10.1016/j.celrep.2021.110152
Cited by 35
AI Summary: OriGene’s pCMV6-TMEM160-Myc-DDK plasmid (Cat# PS100007) was used in this study to investigate the role of Tmem160 in nerve injury-induced neuropathic pain behaviors in male mice.
Snippet: … Here, we demonstrate the significance of the transmembrane protein (Tmem) 160 for nerve injury-induced neuropathic pain. An extensive behavioral assessment Save;HyPer-mito-YFP Kind gift from Prof. Dr. Michael Müller, UMG Goettingen N/A pCDNA3.1-myc-His Invitrogen Cat# V80020 pCMV6-TMEM160-Myc-DDK Origene CAT# PS100007
Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor
Onabajo, Banday, Stanifer et al
Nat Genet (2020) 52 (12), 1283-1293 DOI: 10.1038/s41588-020-00731-9
Cited by 272
AI Summary: OriGene products contributed to this study by providing ACE2 expression plasmids with C-terminal GFP tag (RG208442) and Myc–DDK tag (RC208442), as well as the control vector pCMV6-AC-Myc-DDK, which were used for functional and expression analyses of ACE2 isoforms.
Snippet: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes COVID-19, utilizes angiotensin-converting enzyme 2 (ACE2) for entry into target cells. ACE2 has been proposed as an interferon-stimulated gene (ISG). Thus, interferon-induced variability;ACE2 with a C-terminal GFP tag (RG208442) and a Myc–DDK tag (RC208442) were purchased from Origene. …
Interferons and viruses induce a novel primate-specific isoform dACE2 and not the SARS-CoV-2 receptor ACE2
Onabajo, Banday, Yan et al
bioRxiv (2020)
Cited by 25
AI Summary: OriGene products, specifically the ACE2 (RC208442) and dACE2 (RC228003) cDNA clones, were used in this study to express and characterize the functions of ACE2 isoforms, enabling the discovery that only the novel dACE2, and not ACE2, is induced by interferons and does not facilitate SARS-CoV-2 entry.
FigureFigureFigure
Showing 1-6 of 20 papers.
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