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pCMV6-AC-Myc Mammalian Expression Vector

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SKU
PS100003
pCMV6-AC-Myc, mammalian vector with C-terminal Myc tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
C-Tag Myc
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal Myc-tag.
  • Such clones are the best for detection and purification of the transgene expression using an anti-Myc antibody.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Myc-tagging vector, PS100012.
  • V2_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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Effect of TDP43-CTFs35 on Brain Endothelial Cell Functions in Cerebral Ischemic Injury
Xu, Zhang, Jiang et al
Mol Neurobiol (2022) 59 (7), 4593-4611 DOI: 10.1007/s12035-022-02869-5
Cited by 15
AI Summary: OriGene’s pCMV6-AC-Myc vector (PS100003) encoding mutant mouse TAR DNA-binding protein (Tardbp) C-terminal fragments (CTFs35) was used to overexpress TDP43-CTFs35 in brain endothelial cells, enabling the study of its effects on tight junction protein expression and cell migration in cerebral ischemic injury.
Snippet: Pathological changes in the brain endothelium play an important role in the progression of ischemic stroke and the compromised BBB under ischemic stroke conditions Save;70% confluence, the cells were transfected with the pCMV6-AC-Myc vector (PS100003, OriGene Technologies, Rockville, MD, USA) encoding mutant mouse TAR DNA-binding protein (Tardbp) (NM_145556) C-terminal fragments (CTFs35, 978 bp) for 4.5 h using Lipofectamine 3000 (Thermo Fisher Scientific)
The TDP43 CFTS Affect Brain Endothelial Cell Functions by Regulating YAP and Tight Junction Proteins in Cerebral Ischemic Injury
Xu, Zhang, Jiang et al
Research Square (2021)
Cited by 2
Snippet: uence for transfection. The pCMV6-AC-Myc vector (PS100003, OriGene Technologies, Rockville, MD, USA) encoding mutant mouse TAR DNA binding protein (Tardbp) (NM_145556) C-terminal fragments (CTF35, 978bp) was
LHCGR and ALMS1 defects likely cooperate in the development of polycystic ovary syndrome indicated by double-mutant mice
Yu, Wang, Tao et al
J Genet Genomics (2021) 48 (5), 384-395 DOI: 10.1016/j.jgg.2021.03.014
Cited by 13
AI Summary: In this study, OriGene's ALMS1 shRNA (TG319658) and LHCGR shRNA (TG319658) were used to knock down gene expression in KGN cells, enabling the researchers to investigate the functional roles of ALMS1 and LHCGR mutations in the development of polycystic ovary syndrome (PCOS) phenotypes.
Cip2a/miR-301a feedback loop promotes cell proliferation and invasion of triple-negative breast cancer
Yin, Chen, Luo et al
J Cancer (2019) 10 (24), 5964-5974 DOI: 10.7150/jca.35704
AI Summary: OriGene products pCMV6-E2F1, pCMV6-c-myc, and pCMV6-XL5 empty plasmid were used in this study to investigate the molecular mechanisms underlying the Cip2a/miR-301a feedback loop in triple-negative breast cancer cell proliferation and invasion.
Snippet: pCMV6-E2F1, pCMV6-c-myc, and pCMV6-XL5 empty plasmid was purchased from Origene (Rockville, MD). …miR-301a-expressing retroviruses, Cip2a-expressing retroviruses were purchased from GENECHEM (Shanghai, China). The Cip2a short hairpin RNA (shRNA)
FigureFigureFigure
Iterative, multiplexed CRISPR-mediated gene editing for functional analysis of complex protease gene clusters
Callies, Tadeo, Simper et al
J. Biol. Chem. (2019)
AI Summary: OriGene’s pCMV6-AC-Myc expression plasmids (cat. no. PS100003) were used to express C-terminal Myc-tagged wildtype and mutant variants of mouse Tmprss11 cDNA, enabling functional analysis of specific protease gene variants in the study.
Snippet: pCMV6-AC-Myc expression plasmids (cat. no. PS100003, OriGene Technologies, Rockville, MD) carrying C-terminal Myc-tagged wildtype and mutant variants of mouse Tmprss11 cDNA were synthesized by Blue Heron Biotech (Seattle, WA)
GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease
Chuang, Tsai, Hsueh et al
Sci Adv (2018) 4 (9), eaat5401 DOI: 10.1126/sciadv.aat5401
AI Summary: OriGene products, specifically the pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, and pCMV6-AC-Flag vectors, were used to construct plasmids for expressing tagged proteins (such as PKCθ and AhR) that enabled the study of protein interactions and signaling mechanisms underlying IL-17A induction in autoimmune disease.
Snippet: PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …
FigureFigureFigure
Showing 1-6 of 16 papers.
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