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pCMV6-AN-Myc Mammalian Expression Vector

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SKU
PS100012
pCMV6-AN-Myc, mammalian vector with N-terminal Myc tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag Myc
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal Myc-tag.
  • Such clones are the best for detection and purification of the transgene expression using an anti-Myc antibody.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal Myc-tagging vector, PS100003.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Distinct pathogenic mutations in ARF1 allow dissection of its dual role in cGAS-STING signalling
Lang, Bergner, Zinngrebe et al
EMBO Rep (2025)
Cited by 5
AI Summary: OriGene products MV6-hARF1-myc-FLAG (PS100012), pCMV6-hARF1-Q71L-myc-FLAG, and pCMV6-hARF1-R19C-myc-FLAG were used to express and analyze different ARF1 variants, enabling the study to dissect ARF1's dual role in cGAS-STING signaling and its impact on interferonopathies.
Snippet: MV6-hARF1-myc-FLAG Origene PS100012 pCMV6-hARF1-Q71L-myc-FLAG Hirschenberger et al (2023) N/A pCMV6-hARF1-R19C-myc-FLAG This study N/A
ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling
Hirschenberger, Lepelley, Rupp et al
Nat Commun (2023) 14 (1), 6770 DOI: 10.1038/s41467-023-42150-4
Cited by 55
AI Summary: OriGene’s pCMV6-hARF1-myc-FLAG expression construct (PS100012) was used in this study to investigate the role of ARF1 mutations in regulating STING activation and type I interferon signalling.
Snippet: … Expression constructs and cloning A construct coding for human ARF1 (pCMV6-hARF1-myc-FLAG) was purchased from Origene (PS100012, kindly provided by Michaela Gack, Florida). … Const;A construct coding for human ARF1 (pCMV6-hARF1-myc-FLAG) was purchased from Origene (PS100012, kindly provided by Michaela Gack, Florida)
Aberrant miR-874-3p/leptin/EGFR/c-Myc signaling contributes to nasopharyngeal carcinoma pathogenesis
Luo, Tsai, Hwang et al
J Exp Clin Cancer Res (2022) 41 (1), 215 DOI: 10.1186/s13046-022-02415-0
Cited by 30
AI Summary: OriGene products, specifically the pCMV6-Flag-Lep (leptin expression plasmid) and pCMV6-Flag (empty vector control), were used to manipulate leptin expression in nasopharyngeal carcinoma cell lines, enabling the study of leptin's role in cancer cell growth and signaling pathways.
Snippet: iRNA knockdown, and miRNA mimic/inhibitor experiments The expression plasmids encoding leptin (pCMV6-Flag-Lep) or a control empty vector (pCMV6-Flag) with a C-terminal Myc-DDK tag were purchased from OriGene Technologies. … Two different synthetic;- The expression plasmids encoding leptin (pCMV6-Flag-Lep) or a control empty vector (pCMV6-Flag) with a C-terminal Myc-DDK tag were purchased from OriGene Save Cached
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Chronically elevated branched chain amino acid levels are pro-arrhythmic
Portero, Nicol, Podliesna et al
Cardiovasc Res (2022) 118 (7), 1742-1757 DOI: 10.1093/cvr/cvab207
Cited by 76
AI Summary: In this study, OriGene products including the BCAT2 shRNA plasmid (TL502431) and BCAT2 antibody (TA322126) were used to manipulate and detect BCAT2 expression, enabling the authors to investigate the molecular mechanisms linking elevated branched chain amino acid levels to cardiac arrhythmias.
FigureFigureFigure
DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection
Pattabhi, Knoll, Gale et al
J. Interferon Cytokine Res. (2019) 39 (6), 331-346 DOI: 10.1089/jir.2018.0163
AI Summary: OriGene products, specifically the DHX15 cDNA (RC205914) and expression vectors pCMV6 (PS100012 and PS100014), were used to generate tagged DHX15 proteins for functional studies in this paper investigating DHX15's role in antiviral immune signaling.
Snippet: kit (Stratagene). DHX15 complementary DNA (cDNA;RC205914;OriGene) was cloned into vectors pEFTak and pCMV6 (PS100012 or PS100014;OriGene) for expression as N-terminally Flag- or myc-tagged proteins. DHX15;- kit (Stratagene). DHX15 complementary DNA (cDNA
MIB1 mutations reduce Notch signaling activation and contribute to congenital heart disease
Li, Yu, Liu et al
Clin Sci (Lond) (2018) 132 (23), 2483-2491 DOI: 10.1042/CS20180732
AI Summary: OriGene products, specifically the MIB1 (RC207418) and JAG1 (RC206307) expression plasmids, were used in this study to assess the functional impact of MIB1 mutations on Notch signaling and JAG1 ubiquitination in the context of congenital heart disease.
FigureFigureFigure
Showing 1-6 of 16 papers.
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