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PCMV6-Neo Mammalian Expression Vector

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SKU
PCMV6NEO
Cloning vector pCMV6-Neo, 10 ug circular
  $470.00
In Stock*
Specifications
Specifications
Product Data
E. coli Selection Ampicillin (100 ug/mL)
Download Vector Sequence
Components 1 vial of 10 ug lyophilized pCMV6-Neo vector
Quality Control This vector was sequence verified and restriction-digest confirmed.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
Resources

file-alt Citations

Outer Pore Collapse as a Potential Mechanism of Partial Loss of Pain in Nav1. 7 M899I
Eswaran, Torres-Ortiz, Hautvast et al
bioRxiv (2026)
AI Summary: OriGene contributed to this study by providing the hNav1.7 WT cDNA in a pCMV6Neo vector, which was used to express the wild-type sodium channel in HEK293t cells for functional analyses.
Snippet: HEK293t cells were cultured in Dulbecco’s modified eagle medium F-12 (DMEM-F12;101 Thermo Fischer Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine 102 serum (FBS good;PAN-Biotech, Aidenbach, Germany) and incubated at 37°C with 5% 103 CO2. The hNav1.7 WT cDNA was contained in a pCMV6Neo vector (Origene, Rockville, 104 MD, USA)
The immortality mechanism of TERT promoter mutant cancers is self-reinforcing and reversible
Stevers, Kim, Yuan et al
Mol Cell (2025) 85 (12), 2337-2354.e9 DOI: 10.1016/j.molcel.2025.05.026
Cited by 5
AI Summary: OriGene’s pCMV6-Neo vector (cat# PCMV6NEO) was used as a source of the CMV enhancer/promoter for constructing reporter plasmids to compare knockdown efficiency in the study.
Snippet: d followed by subsequent cloning of other promoters for comparison of knockdown efficiency. Next, the pCMV6-Neo vector (Origene cat# PCMV6NEO) was digested with FastDigest Bsu15I and FastDigest XmaJI to release the CMV enhancer/promoter for ligation into the N174 -TurboGFP-miR-E-puro backbone. Finally, N174-CMV-GFP-miR-E-puro vector was digeste
The Immortality Mechanism of TERT Promoter Mutant Cancers Is Self-Reinforcing and Reversible by Targeted Degradation
Stevers
Thesis (2024)
Snippet: ng of other promoters for comparison of knockdown efficiency. Next, the pCMV6-Neo vector (Origene Cat# PCMV6NEO) was digested with FastDigest Bsu15I and FastDigest XmaJI to release the CMV enhancer/promoter for ligation into the N174 - TurboGFP-miR-E-puro backbone. Finally, N174-CMV-G
A unified and self-reinforcing mechanism of tumor cell immortality
Stevers, Barger, Yuan et al
Research Square (2023)
Snippet: The EF1a core promoter was first introduced followed by subsequent cloning of other promoters for comparison of knockdown efficiency. Next, the pCMV6-Neo vector (Origene Cat# PCMV6NEO) was digested with FastDigest Bsu15I and FastDigest XmaJI to release the CMV enhancer/promoter for ligation into the N174 -TurboGFP-miR
Myeloid-derived suppressor cells (MDSCs) depletion by cabozantinib improves the efficacy of anti-HER2 antibody-based immunotherapy in a 4T1-HER2 murine breast cancer model
Khaki Bakhtiarvand, Ramezani-Ali Akbari, Amir Jalali et al
Int Immunopharmacol (2022) 113 (Pt B), 109470 DOI: 10.1016/j.intimp.2022.109470
Cited by 20
AI Summary: OriGene’s pCMV6-neo-HER2 construct was used to generate HER2-positive 4T1 breast cancer cells, enabling the study of anti-HER2 immunotherapy efficacy in the murine model.
Snippet: Background Clinical trials using Cabozantinib have shown promising results in metastatic breast cancer. This efficacy mainly results from removing and/or polarization of Save;In brief, 25 × 104 4T1 murine breast cancer cells were cultured in a six-well culture plate overnight at 37 °C. pCMV6-neo-HER2 construct (OriGene Technologies) and jetPEI (Polyplus-transfection, France) were separately dilu...
Immunisation Using Novel DNA Vaccine Encoding Virus Membrane Fusion Complex and Chemokine Genes Shows High Protection from HSV-2
Gompels, Bravo, Briggs et al
Viruses (2022) 14 (11), 2317 DOI: 10.3390/v14112317
AI Summary: OriGene’s pCMV6neo plasmid was used as the DNA expression vector for vaccine constructs in this study, enabling efficient expression of viral and chemokine genes for immunisation experiments.
Snippet: ech/Eurofins, Bothell, DC, USA), sequence verified and expressed from plasmid pCMV6neo containing human CMV transcriptional 5′ enhancer, promoter and start site plus SV40 3′ polyadenylation site (Origene Technologies Inc., Rockville, MD, USA). 2.2. Animals and Virus Female Hartley guinea pigs (Charles River Laboratories, Wilmington, NC, USA), pathogen free, were used (250–350 g), and housed
Showing 1-6 of 62 papers.
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